UniProt Accession

 ERF3B_MOUSE; Q149F3; O88180; Q9CY91 

Genbank Protein ID

 AK019241; AL645466; BC117825; BC117826; AB003503 

Genbank Nucleotide ID

 BAB31621.1; CAM15955.1; AAI17826.1; AAI17827.1; BAA32527.1 

Protein Name

 Eukaryotic peptide chain release factor GTP-binding subunit ERF3B 

Protein Synonyms/Alias

 Eukaryotic peptide chain release factor subunit 3b; eRF3b; G1 to S phase transition protein 2 homolog 

Gene Name

 Gspt2 

Gene Synonyms/Alias

 Erf3b 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
285	AYFETEKKHFTILDA	acetylation	[1]
329	EFETGFEKGGQTREH	acetylation	[2]
329	EFETGFEKGGQTREH	ubiquitination	[3]
373	RYEECKEKLVPFLKK	acetylation	[4]
373	RYEECKEKLVPFLKK	ubiquitination	[3]
442	IRLPIVDKYKDMGTV	acetylation	[1]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Involved in translation termination in response to the termination codons UAA, UAG and UGA. May play a role as a potent stimulator of the release factor activity of ETF1. Exhibits GTPase activity, which is ribosome- and ETF1-dependent. May play a role in cell cycle progression. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (By similarity). 

Sequence Annotation

 NP_BIND 214 221 GTP (By similarity).
 NP_BIND 291 295 GTP (By similarity).
 NP_BIND 353 356 GTP (By similarity).  

Keyword

 Cell cycle; Complete proteome; Cytoplasm; GTP-binding; Nonsense-mediated mRNA decay; Nucleotide-binding; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 632 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
 GO:0006412; P:translation; IEA:UniProtKB-KW. 

Interpro

 IPR009818; Ataxin-2_C.
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR009001; Transl_elong_EF1A/Init_IF2_C.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR004160; Transl_elong_EFTu/EF1A_C.
 IPR009000; Transl_elong_init/rib_B-barrel. 

Pfam

 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF03143; GTP_EFTU_D3
 PF07145; PAM2 

SMART

  

PROSITE

 PS00301; EFACTOR_GTP 

PRINTS

 PR00315; ELONGATNFCT.