CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029253
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Karst, isoform E 
Protein Synonyms/Alias
  
Gene Name
 kst 
Gene Synonyms/Alias
 CG12008; Dmel_CG12008 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
1850AKQQLIEKMLKSLHKacetylation[1]
2044DSVRLQAKYPSNASAacetylation[1]
2803DADLIREKLAYILRRacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4337 AA 
Protein Sequence
MEYNSVLRSN FSRNEYRRYI SYERQSLASQ YEPGGYSALQ TPTPSNRNSA NMTQRDGIIK 60
FENERIKTLQ EERLHIQKKT FTKWMNSFLI KAKMEVEDLF TDLADGIKLL KLLEIISSEK 120
LGKPNSGRMR VHKIENVNKS LAFLHTKVRL ESIGAEDIVD GNPRLILGLI WTIILRFQIQ 180
EIEIDVDEEN ESSEKRSAKD ALLLWCQRKT HGYPGVNITD FTNSWRSGLG FNALIHSHRP 240
DLFEYSTIVN SKNSNLDNLN HAFDTAANEL GIPSLLDAED IDSARPDEKS ILTYVASYYH 300
TFARMKNEQK SGKRIANIVG QLMDADRKKM QYEGLTTNLL SWIRQKTLEL EQRDLPNSLE 360
GIQRELLAFK EYRTIEKPPK YKERSEIEAL YFTINTLLKA LNQPPYNPQD GQLVNDIEKA 420
WQILEYAEHH REVALRDELL RQEKLEQLNY KFEKKSVLRE GYLKEMIQVL SDPRYLRQVD 480
ATLKKHEAIS ADILARVERF NDLTAMAEEL DRENYHGKER VRRREQEVMA KWRQLLELLE 540
NQRLNLSQMS NLMNLLREIA STTEAVRELQ QQFASEDVGP HLLGVEELLQ AHSLQELQVN 600
TYGETLKRFN RQALPYKSSE HKDAALLAQR LADLEEAYSE LLRRSAARRA RLEEARNFHH 660
FMEDYDNEES WLVDKQRICK TGITAKDLRA VLSLQQKHKA LEDEIKSRKP KSGQMSTAGK 720
RLIGEQHPRS SEIQSRIDSL AEHWQALEAL VELRRRQLED AAEAYQFYTD ANEAESWLNE 780
KIALVNSRDY GNDEPSAQAL LQRHRDLQGE LNAYSGDILN LNQQADKLIK AGICTLELSA 840
AEPELPEVEQ EEWVNETRLV PKEVWEDEWV EKLEHKKVTE TKMLPHVKSL FPFEGQGMKM 900
DKGEVMLLKS KTNDDWWCVR KDNGVEGFVP ANYVREVEPR PVACIVPKAE KVKSLQKVKK 960
TILVRQVVPV KRIKPVSVAP KPLVQRRTST QSINENADSV EKRQQRINQT YDELQEMAQK 1020
RHALLEDSIH LFGFYRECDD FEKWMKEKER MIKSDEGEGV DNAKRKFEKF ITDLSAASKR 1080
VEEIDGAVDT FRRQGHSQLD KIIARQRQIH QIWQRLNNAK AQREKSLEGA SSVELFNRTC 1140
DEAKVWMSEK MLQLDTAVIT PDLRTVQALQ RRHQNLEREL APVEDKVNRV TYLGNSVKNA 1200
YPAEKDNVNA RQQEVQDMWQ QVQQRGSDLR NRIESEVGQQ VFNNSAKVLL AWIDSVKDQL 1260
NADESARDVE TANNLLKKHN DLGDDIRAHD TEFVEVIQLG KQLSDGKPNM AETVAVIERL 1320
KAEQDAIHRG WAEKQKWLLQ CVDLQMFNRE ADKIDATTKS HEAFLEYNNL GASLDEVEAI 1380
LKRHLDFEKS LMAQDKILKG FSDNADKLIS NDHYDSKYIG DRRNQVLGKR KAVKDRAFER 1440
KRLLQASKDF HKFAAEADDL KVWLQDKTRI AGDENYRDLS NLPRKLQKHQ AFERELRANE 1500
GQLRNVTKDG QALVQAGNRV PEVESRVADL NKRWKDLLTL SEDKGRKLEQ AASQREHNRS 1560
LEDAKKKVDE LDSALRSGDV GNDLRSCKDL INKQQILESE ITIWDQKVAE LVSTGDDMAH 1620
GGHFNAQNIE AGTKELQQRF KDLRDPTQRR RAKLEESLNY HKFVFELDSE FQWINEHLPA 1680
AKSNELGQNL HQAQSLHKKH KKLEAEIKGH QPMINKALVA GQSLISQQHP EREQVESLCQ 1740
QLEQAWQDLE RHCGERSRKL DMSLKAQQYL FDAGEIESWL GERNNVLRST EYGRDRDSAA 1800
KLLTKHKTIE LELDTYSGIV TEMGHSCAAM VAANHPDSKV LAAKQQLIEK MLKSLHKLAS 1860
QRQGRLMESL YKHEYFLESD EVEQWIREQE QAASSEDYGQ DFEHLQLLQN KFDDLKHRVE 1920
VGADRVDQCE LLAKKLIDSE SPYANEVEKR QEQLRTSWEN LLQLLNQREQ KLHAAGEIHR 1980
FHRDVAEALF RIQDKNAALS QELGRDLNSA LALLRKHEGF ENDLVALEAQ LQVLVEDSVR 2040
LQAKYPSNAS AIAQQQDKVV AAWNDLKERS TARGDRLAAS SDLQTFLTDV RDIVSWSSNL 2100
RAALQAEEHV SDAAGATALK IQHDAIYGEI EAREDKFRYL NELSDSMVQT GHYAAADVEE 2160
KCAAMLDERQ KLHAAWNKKK IMLEQKIDLF CFLRDAKQID NLSSSQQAAL SSSDFGQTVE 2220
DVQNKIRKHD EFERLIQTQE EKVSLLQEHG RKLIEQRHYD SANIQTILQG VLARRQKVKD 2280
LCAVRRYKLE DALLYAKFVR DCAEAKYWIN EKQKKLEADA ASYAEVTNLD EKIKKLQKHQ 2340
AFQAEVAANQ GRIQEIQDTG VILLSKQHES SPEIKRAIEI VLEAWQGLLA ELEQRGRGLE 2400
EAQDSLEFNS QLDKIEAWIR DKEMMVQASD TGRDLEHCNA LMRKLDDVDS DMRVDDQRVK 2460
HINQLADKLI NQAQVPADTQ SVDKRRKDFN YNWRQLQGAL NAYRALLGGA NEIHVFNRDV 2520
DDTADRIAEK SLAMSSTDTG RDLAAVEALI RREEALERDM SAVKQKIDQH ETAAEFLIKK 2580
YPERGAQHIE RKLEELHKSW GNLQALSVKR QSILNEAYLA HKFVSDVKEL ELWVNDMIKK 2640
MNNTQSPSTI NDCETQLELH QERKVEIEGR QEAFAGLKQQ GEQLSKRPQQ QQPDNVRKYL 2700
LVLEELHQTL NEAWSERARD LTEAHQLQLF KAQVEQVEIW LANKEAFLNN DDLGDSYTAV 2760
ERLLKKHDEF EKLLHADHVD TLQKFANSIL EGEPKDADLI REKLAYILRR KQKLLELSEE 2820
RKQRLTQSHQ LQEFLRSLYE IDRWLVQKLQ VALDENYREP SNLQSKIQKH AAFDAELLSN 2880
SPRVQSVIHE GERLIRGDHF AKDEIAQQVQ LLEGDWLKLK GASQTKKDKL QQAYDALAFN 2940
RSVDEFNNWM DEVELQLSSE DYGKDLAAVS NLLKKHERLE ADVAHHGELA DQLKQKDEQF 3000
FQAEHFLRHE IHERATVSIR RYNTLHEPLG IRRENLEDSL SLQQFLRDAE DELQWLAEKQ 3060
LVAGSQDLGT SLLSVQGLQK KHNSLEAELT SQEPLIQALL QRGQQMIRDN HFASEQLQYK 3120
SELLQKQLVQ LRDLAAIRRL RLLDAVESQL FYVEANEADA WMREKRPVLS SSDYGRDEVS 3180
VQGHQKKLEV LQRELTAFKP SIEKVAKLAT GLIERNHFDS SNIAEKNAQV GQEYEDLLRL 3240
AKERESRLGE CKKLFEYLRE TEELHEWVGD QMAVTASEDY GEDVEHVEQL ILAFESFVSN 3300
LNANEARVEA CLERGDRLIQ ENNPYRSSIK SKRDETKQLW EELKDLVHAR QDALAGAKQV 3360
HVYDRVADET IQLINEKDAS LISEDYGQDL ESIQALGRKH QVFESELVGI QGQVDSVLAE 3420
AAKLGEIYPD AKEHIEVKRD ETVEAWTDLK EKTAARKNKL SQAEQLQSYF DEYRDLIAWI 3480
NEMLAKITAP ELANSVAGAE LLLASTKDHD TEIRTRDETF AKFAANGQQL IKEKHFLAHE 3540
VEDKIKVLQA RHELLKHTLN KRREIYELNL DTQLFLKDAE ILEQWISSRE PQLKDTKLGD 3600
SIPQVEDLLR RHEDFEKTVA AQEEKFQAIK RITMLEQLFR HQLEQEKISK LQEKERLEKE 3660
RLEQLKQREL QRLADERRRA EKQHEHRQNA ASQEKTPIFS SPMVTPAQTS GPQSPALSQV 3720
QLRPPFGDDN EHLALQKSSS SGMFGDRLRR GSADANVKRA ESMKVQPKQA KRTPSFTTRR 3780
RAQSFRKNQK GEGFDLPPVE IQGSLERKHG LQSGGKKAPV RSWKQFHTVL CGQLVCFFKD 3840
ENDFLQQKTA TAPVNILGAK CERADDYTKK KYVFRLKLPD GSEFLFEAPS LDILNDWVRK 3900
ISFHASLPPN MQLLSYDESM KQQSSSSPDI KVTSSVESPV SSRNSSPDSQ RRTSGAQVLD 3960
GTATPQMAFL QRQMQQQQQQ QQSQPSSPTG GFDQKPPIPP RGAAPVASHR QSQENLVVMR 4020
NRQSSNDLQQ SATLPAGLTG VQQNGNGKDD NALLTRNSEA RQSDNPPPLP TTMPPVGGQH 4080
QHPQNSHSHQ NQHQAQVQQR INAFNAAASQ QHQPDYFNNN TARQQPQRIP SGRIDSTRKF 4140
IEMEAHNNNG GTSSSPKRST INYSSSGASS NGNGNVKIGS GNSSTTTITT STTTHQVTSS 4200
SRTVWHLTSS PTSSTKSSST GGSGEPSHAI SNPSYMGLHL NNNNDSIGIG LGGWGNTRFE 4260
SNRPVSLQPD SISFSRVSAE SSSESEAQSI SSVSGVKGSK GTKEERRSGM FRIFGRKGDK 4320
EKEKDKDKRR SSQVPPQ 4337 
Gene Ontology
 GO:0005912; C:adherens junction; IDA:FlyBase.
 GO:0016324; C:apical plasma membrane; IDA:FlyBase.
 GO:0016327; C:apicolateral plasma membrane; TAS:FlyBase.
 GO:0008091; C:spectrin; ISS:FlyBase.
 GO:0003779; F:actin binding; IDA:FlyBase.
 GO:0008017; F:microtubule binding; IDA:FlyBase.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0000910; P:cytokinesis; IMP:FlyBase.
 GO:0006897; P:endocytosis; IMP:FlyBase.
 GO:0030707; P:ovarian follicle cell development; TAS:FlyBase.
 GO:0007009; P:plasma membrane organization; TAS:FlyBase.
 GO:0010797; P:regulation of multivesicular body size involved in endosome transport; IMP:FlyBase.
 GO:0042060; P:wound healing; IMP:FlyBase.
 GO:0045186; P:zonula adherens assembly; TAS:FlyBase. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR001605; PH_dom-spectrin-type.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF00169; PH
 PF07653; SH3_2
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00233; PH
 SM00326; SH3
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00683; SPECTRINPH.