UniProt Accession

 PFLA_ECOLI; P0A9N4; P09374 

Genbank Protein ID

 X08035; U00096; AP009048 

Genbank Nucleotide ID

 CAA30829.1; AAC73988.1; BAA35637.1 

Protein Name

 Pyruvate formate-lyase 1-activating enzyme 

Protein Synonyms/Alias

 Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 

Gene Name

 pflA 

Gene Synonyms/Alias

 act; b0902; JW0885 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
49	TWDTHGGKEVTVEDL	acetylation	[1]
58	VTVEDLMKEVVTYRH	acetylation	[1]
155	HRTLEFAKYLANKNV	acetylation	[1]
163	YLANKNVKVWIRYVV	acetylation	[1]
196	RDMGNVEKIELLPYH	acetylation	[1]
207	LPYHELGKHKWVAMG	acetylation	[1]
209	YHELGKHKWVAMGEE	acetylation	[1]
243	ILEQYGHKVMF****	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine. 

Sequence Annotation

 METAL 30 30 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 34 34 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 37 37 Iron-sulfur (4Fe-4S-S-AdoMet) (By  

Keyword

 3D-structure; 4Fe-4S; Carbohydrate metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Glucose metabolism; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Reference proteome; S-adenosyl-L-methionine. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 246 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0043365; F:[formate-C-acetyltransferase]-activating enzyme activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016491; F:oxidoreductase activity; IDA:EcoliWiki.
 GO:0006006; P:glucose metabolic process; IEA:UniProtKB-KW.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki. 

Interpro

 IPR006638; Elp3/MiaB/NifB.
 IPR012838; PFL_activating.
 IPR001989; Radical_activat_CS.
 IPR007197; rSAM. 

Pfam

 PF04055; Radical_SAM 

SMART

 SM00729; Elp3 

PROSITE

 PS01087; RADICAL_ACTIVATING 

PRINTS