UniProt Accession

 KAPR_YEAST; P07278; D6VVP9 

Genbank Protein ID

 M15756; M17223; X05051; Z46861; AY558087; BK006942 

Genbank Nucleotide ID

 AAA34468.1; AAA66934.1; CAA28726.1; CAA86918.1; AAS56413.1; DAA08515.1 

Protein Name

 cAMP-dependent protein kinase regulatory subunit 

Protein Synonyms/Alias

 cAPK regulatory subunit; Bypass of cyclase mutations protein 1; Protein kinase A regulatory subunit; PKA regulatory subunit 

Gene Name

 BCY1 

Gene Synonyms/Alias

 REG1; SRA1; YIL033C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
57	REPEFKAKNIVLFPE	ubiquitination	[1]
88	SRSSVMFKSPFVNED	acetylation	[2]
102	DPHSNVFKSGFNLDP	acetylation	[2]
304	LKSMPVLKSLTTYDR	ubiquitination	[3]
313	LTTYDRAKLADALDT	acetylation	[2]
321	LADALDTKIYQPGET	acetylation	[2]

Reference

 [1] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1. 

Sequence Annotation

 NP_BIND 184 301 cAMP 1.
 NP_BIND 302 416 cAMP 2.
 REGION 2 183 Dimerization and phosphorylation.
 REGION 8 45 Dimerization/docking domain (D/D).
 MOTIF 142 146 Inhibitor sequence (IS).
 BINDING 249 249 cAMP 1.
 BINDING 258 258 cAMP 1.
 BINDING 368 368 cAMP 2.
 BINDING 377 377 cAMP 2.
 MOD_RES 3 3 Phosphoserine.
 MOD_RES 4 4 Phosphoserine.
 MOD_RES 9 9 Phosphoserine.
 MOD_RES 68 68 Phosphoserine.
 MOD_RES 70 70 Phosphoserine.
 MOD_RES 74 74 Phosphoserine.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 79 79 Phosphoserine.
 MOD_RES 81 81 Phosphoserine.
 MOD_RES 83 83 Phosphoserine.
 MOD_RES 84 84 Phosphoserine.
 MOD_RES 129 129 Phosphothreonine.
 MOD_RES 130 130 Phosphoserine.
 MOD_RES 131 131 Phosphothreonine.
 MOD_RES 144 144 Phosphothreonine.
 MOD_RES 145 145 Phosphoserine; by autocatalysis.
 MOD_RES 147 147 Phosphoserine.
 MOD_RES 150 150 Phosphothreonine.  

Keyword

 3D-structure; cAMP; cAMP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 416 AA 

Protein Sequence

Gene Ontology

 GO:0005952; C:cAMP-dependent protein kinase complex; IEA:InterPro.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
 GO:0004862; F:cAMP-dependent protein kinase inhibitor activity; IDA:SGD.
 GO:0008603; F:cAMP-dependent protein kinase regulator activity; IEA:InterPro.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IDA:SGD.
 GO:0007165; P:signal transduction; IEA:InterPro. 

Interpro

 IPR002373; cAMP/cGMP_kin.
 IPR012198; cAMP_dep_PK_reg_su.
 IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
 IPR018490; cNMP-bd-like.
 IPR018488; cNMP-bd_CS.
 IPR000595; cNMP-bd_dom.
 IPR014710; RmlC-like_jellyroll. 

Pfam

 PF00027; cNMP_binding
 PF02197; RIIa 

SMART

 SM00100; cNMP
 SM00394; RIIa 

PROSITE

 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3 

PRINTS

 PR00103; CAMPKINASE.