UniProt Accession

 EI2BA_YEAST; P14741; D6VX91 

Genbank Protein ID

 M23356; Z28251; BK006944 

Genbank Nucleotide ID

 AAA34637.1; CAA82098.1; DAA09181.1 

Protein Name

 Translation initiation factor eIF-2B subunit alpha 

Protein Synonyms/Alias

 GCD complex subunit GCN3; Guanine nucleotide exchange factor subunit GCN3; Transcriptional activator GCN3; eIF-2B GDP-GTP exchange factor subunit alpha 

Gene Name

 GCN3 

Gene Synonyms/Alias

 AAS2; TIF221; YKR026C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
37	LVTLLRIKTPETAAE	ubiquitination	[1]
225	VLAHNARKPFYVVTE	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)- bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally. 

Sequence Annotation

 MOD_RES 291 291 Phosphothreonine.  

Keyword

 Activator; Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; Repressor; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 305 AA 

Protein Sequence

Gene Ontology

 GO:0005851; C:eukaryotic translation initiation factor 2B complex; IDA:SGD.
 GO:0032045; C:guanyl-nucleotide exchange factor complex; IDA:SGD.
 GO:0030234; F:enzyme regulator activity; IMP:SGD.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0006446; P:regulation of translational initiation; IDA:SGD. 

Interpro

 IPR000649; IF-2B-related. 

Pfam

 PF01008; IF-2B 

SMART

  

PROSITE

  

PRINTS