CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012480
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 IQ calmodulin-binding motif-containing protein 1 
Protein Synonyms/Alias
 Nephrocystin-5; p53 and DNA damage-regulated IQ motif protein; PIQ 
Gene Name
 IQCB1 
Gene Synonyms/Alias
 KIAA0036; NPHP5; OK/SW-cl.85 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19SIAAEVAKSPEQNVPubiquitination[1]
31NVPVILLKLKEIINIubiquitination[1, 2]
33PVILLKLKEIINITPubiquitination[1]
48LGSSELKKIKQDIYCubiquitination[1]
205DLLRIGRKALYSILDubiquitination[3, 4, 5]
263GLRRLLSKQETGTEFubiquitination[1, 2]
331LQRSFRSKRSKMLLEmethylation[6]
334SFRSKRSKMLLEINRubiquitination[1]
528LMKAPSLKEAEGKEPubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510] 
Functional Description
 Involved in ciliogenesis (By similarity). 
Sequence Annotation
 DOMAIN 294 317 IQ 1.
 DOMAIN 318 338 IQ 2.
 DOMAIN 387 416 IQ 3.
 DOMAIN 417 437 IQ 4.
 MOD_RES 572 572 Phosphoserine.  
Keyword
 Alternative splicing; Calmodulin-binding; Ciliopathy; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Nephronophthisis; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Senior-Loken syndrome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 598 AA 
Protein Sequence
MKPTGTDPRI LSIAAEVAKS PEQNVPVILL KLKEIINITP LGSSELKKIK QDIYCYDLIQ 60
YCLLVLSQDY SRIQGGWTTI SQLTQILSHC CVGLEPGEDA EEFYNELLPS AAENFLVLGR 120
QLQTCFINAA KAEEKDELLH FFQIVTDSLF WLLGGHVELI QNVLQSDHFL HLLQADNVQI 180
GSAVMMMLQN ILQINSGDLL RIGRKALYSI LDEVIFKLFS TPSPVIRSTA TKLLLLMAES 240
HQEILILLRQ STCYKGLRRL LSKQETGTEF SQELRQLVGL LSPMVYQEVE EQKLHQAACL 300
IQAYWKGFQT RKRLKKLPSA VIALQRSFRS KRSKMLLEIN RQKEEEDLKL QLQLQRQRAM 360
RLSRELQLSM LEIVHPGQVE KHYREMEEKS ALIIQKHWRG YRERKNFHQQ RQSLIEYKAA 420
VTLQRAALKF LAKCRKKKKL FAPWRGLQEL TDARRVELKK RVDDYVRRHL GSPMSDVVSR 480
ELHAQAQERL QHYFMGRALE ERAQQHREAL IAQISTNVEQ LMKAPSLKEA EGKEPELFLS 540
RSRPVAAKAK QAHLTTLKHI QAPWWKKLGE ESGDEIDVPK DELSIELENL FIGGTKPP 598 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0032391; C:photoreceptor connecting cilium; IDA:HGNC.
 GO:0001750; C:photoreceptor outer segment; IEA:Compara.
 GO:0005516; F:calmodulin binding; IDA:HGNC.
 GO:0042384; P:cilium assembly; ISS:UniProtKB.
 GO:0048496; P:maintenance of organ identity; IMP:HGNC.
 GO:0045494; P:photoreceptor cell maintenance; IMP:HGNC. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS. 
Pfam
 PF00612; IQ 
SMART
 SM00015; IQ 
PROSITE
 PS50096; IQ 
PRINTS