CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037010
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Plasma membrane calcium-transporting ATPase 1 
Protein Synonyms/Alias
  
Gene Name
 ATP2B1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67IENRNKAKAQDGAAMubiquitination[1, 2]
80AMEMQPLKSEEGGDGubiquitination[1, 2, 3]
90EGGDGDEKDKKKANLubiquitination[1, 3]
195YSVKKMMKDNNLVRHubiquitination[1, 4]
238VQAYINEKHYKKVPEacetylation[5]
241YINEKHYKKVPEPEAacetylation[5]
276SKILPPEKEGGLPRHubiquitination[4, 6]
300LGLLLDLKRDYQDVRubiquitination[3]
365LSANGEAKVFRPRDRubiquitination[6]
377RDRDDIVKTVIEPMAubiquitination[1, 2, 6]
490NRRIRNEKGEIEQERubiquitination[2, 4]
500IEQERIDKIWPKLRVubiquitination[6]
549TNDGPALKKADVGFAubiquitination[1, 2, 4, 6, 7]
583DNFTSIVKAVMWGRNubiquitination[2]
664RKPYGRNKPLISRTMubiquitination[1, 4]
877SLYEGLEKPESRSSIubiquitination[6, 8]
917AEDDAPTKRNSSPPPubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Hydrolase; Membrane; Nucleotide-binding; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 963 AA 
Protein Sequence
MFLVIGTHVM EGSGRMVVTA VGVNSQTGII FTLLGAGGEE EEKKDEKKKE KKNKKQDGAI 60
ENRNKAKAQD GAAMEMQPLK SEEGGDGDEK DKKKANLPKK EKSVLQGKLT KLAVQIGKAG 120
LLMSAITVII LVLYFVIDTF WVQKRPWLAE CTPIYIQYFV KFFIIGVTVL VVAVPEGLPL 180
AVTISLAYSV KKMMKDNNLV RHLDACETMG NATAICSDKT GTLTMNRMTV VQAYINEKHY 240
KKVPEPEAIP PNILSYLVTG ISVNCAYTSK ILPPEKEGGL PRHVGNKTEC ALLGLLLDLK 300
RDYQDVRNEI PEEALYKVYT FNSVRKSMST VLKNSDGSYR IFSKGASEII LKKCFKILSA 360
NGEAKVFRPR DRDDIVKTVI EPMASEGLRT ICLAFRDFPA GEPEPEWDNE NDIVTGLTCI 420
AVVGIEDPVR PEVPDAIKKC QRAGITVRMV TGDNINTARA IATKCGILHP GEDFLCLEGK 480
DFNRRIRNEK GEIEQERIDK IWPKLRVLAR SSPTDKHTLV KGIIDSTVSD QRQVVAVTGD 540
GTNDGPALKK ADVGFAMGIA GTDVAKEASD IILTDDNFTS IVKAVMWGRN VYDSISKFLQ 600
FQLTVNVVAV IVAFTGACIT QDSPLKAVQM LWVNLIMDTL ASLALATEPP TESLLLRKPY 660
GRNKPLISRT MMKNILGHAF YQLVVVFTLL FAGEKFFDID SGRNAPLHAP PSEHYTIVFN 720
TFVLMQLFNE INARKIHGER NVFEGIFNNA IFCTIVLGTF VVQIIIVQFG GKPFSCSELS 780
IEQWLWSIFL GMGTLLWGQL ISTIPTSRLK FLKEAGHGTQ KEEIPEEELA EDVEEIDHAE 840
RELRRGQILW FRGLNRIQTQ IRVVNAFRSS LYEGLEKPES RSSIHNFMTH PEFRIEDSEP 900
HIPLIDDTDA EDDAPTKRNS SPPPSPNKNN NAVDSGIHLT IEMNKSATSS SPGSPLHSLE 960
TSL 963 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005388; F:calcium-transporting ATPase activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:InterPro. 
Interpro
 IPR022141; ATP_Ca_trans_C.
 IPR006408; ATPase_P-typ_Ca-transp_plasma.
 IPR006068; ATPase_P-typ_cation-transptr_C.
 IPR023299; ATPase_P-typ_cyto_domN.
 IPR018303; ATPase_P-typ_P_site.
 IPR023298; ATPase_P-typ_TM_dom.
 IPR008250; ATPase_P-typ_transduc_dom_A.
 IPR001757; Cation_transp_P_typ_ATPase.
 IPR023214; HAD-like_dom. 
Pfam
 PF12424; ATP_Ca_trans_C
 PF00689; Cation_ATPase_C
 PF00122; E1-E2_ATPase
 PF00702; Hydrolase 
SMART
  
PROSITE
 PS00154; ATPASE_E1_E2 
PRINTS
 PR00119; CATATPASE.