CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013455
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Acylglycerol kinase, mitochondrial 
Protein Synonyms/Alias
 hAGK; Multiple substrate lipid kinase; HsMuLK; MuLK; Multi-substrate lipid kinase 
Gene Name
 AGK 
Gene Synonyms/Alias
 MULK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
6**MTVFFKTLRNHWKacetylation[1]
6**MTVFFKTLRNHWKubiquitination[2]
107TDYEGQAKKLLELMEubiquitination[3]
199VLQIKGEKEQPVFAMubiquitination[3]
223DAGVKVSKYWYLGPLubiquitination[2]
327NQLDPTSKEDFLNICubiquitination[3, 4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. 
Sequence Annotation
 DOMAIN 58 199 DAGKc.  
Keyword
 Alternative splicing; ATP-binding; Cardiomyopathy; Cataract; Complete proteome; Kinase; Membrane; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 422 AA 
Protein Sequence
MTVFFKTLRN HWKKTTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFG NQLIPPNAQV 60
KKATVFLNPA ACKGKARTLF EKNAAPILHL SGMDVTIVKT DYEGQAKKLL ELMENTDVII 120
VAGGDGTLQE VVTGVLRRTD EATFSKIPIG FIPLGETSSL SHTLFAESGN KVQHITDATL 180
AIVKGETVPL DVLQIKGEKE QPVFAMTGLR WGSFRDAGVK VSKYWYLGPL KIKAAHFFST 240
LKEWPQTHQA SISYTGPTER PPNEPEETPV QRPSLYRRIL RRLASYWAQP QDALSQEVSP 300
EVWKDVQLST IELSITTRNN QLDPTSKEDF LNICIEPDTI SKGDFITIGS RKVRNPKLHV 360
EGTECLQASQ CTLLIPEGAG GSFSIDSEEY EAMPVEVKLL PRKLQFFCDP RKREQMLTSP 420
TQ 422 
Gene Ontology
 GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
 GO:0047620; F:acylglycerol kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0001729; F:ceramide kinase activity; IEA:Compara.
 GO:0004143; F:diacylglycerol kinase activity; IEA:EC.
 GO:0046513; P:ceramide biosynthetic process; IEA:Compara.
 GO:0046486; P:glycerolipid metabolic process; IEA:UniProtKB-UniPathway.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR001206; Diacylglycerol_kinase_cat_dom. 
Pfam
 PF00781; DAGK_cat 
SMART
 SM00046; DAGKc 
PROSITE
 PS50146; DAGK 
PRINTS