CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013654
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine--tRNA ligase, mitochondrial 
Protein Synonyms/Alias
 Tyrosyl-tRNA synthetase; TyrRS 
Gene Name
 Yars2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
349RLAAEVTKLVHGQEGacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) (By similarity). 
Sequence Annotation
 MOTIF 76 85 "HIGH" region.
 MOTIF 275 279 "KMSKS" region.
 BINDING 278 278 ATP (By similarity).
 MOD_RES 349 349 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Mitochondrion; Nucleotide-binding; Protein biosynthesis; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 471 AA 
Protein Sequence
MAAPMLRHLC RVPQSGVWTR GPRAVRPGAR GMLVAPRARG LFKEFFPESG TKTELPELFD 60
RRRAGSPQTV YCGFDPTGDS LHVGHLLTLL GLFHFQRAGH NVIALVGGST ALLGDPSGRT 120
KEREALSAEC VRANARALQR GLETLAANHA RLFADGRPWG TFTVLDNAAW FQKQHLMDFL 180
ATVGGHFRMG TLLSRLSVQS RLKSPEGMSL AEFFYQVLQA YDFYYLFRHY GCRVQLGGSD 240
QLGNIMSGYE FIHKLTGEDV FGITVPLITS TTGAKLGKSA GNAVWLNREK TSPFELYQFF 300
IRQQDDSVER YLKLFTFLPL PEIDHIMQLH VKEPEKRIAQ KRLAAEVTKL VHGQEGLDSA 360
KRCTQALYHS SIEALEVMSD QELKELFKEA SFSELVLDPG TSVIDTCRKA NAIPDGPRGY 420
RMITEGGVSI NHRQVTNPES VLVIGQHILK NGLSLLKIGK RNFYIIKWLQ L 471 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0072545; F:tyrosine binding; IEA:Compara.
 GO:0004831; F:tyrosine-tRNA ligase activity; IEA:EC.
 GO:0006437; P:tyrosyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR001412; aa-tRNA-synth_I_CS.
 IPR002305; aa-tRNA-synth_Ic.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR002942; S4_RNA-bd.
 IPR002307; Tyr-tRNA-ligase.
 IPR024088; Tyr-tRNA-ligase_bac-type. 
Pfam
 PF00579; tRNA-synt_1b 
SMART
  
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR01040; TRNASYNTHTYR.