UniProt Accession

 CLIC4_MOUSE; Q9QYB1; Q8BMG5 

Genbank Protein ID

 AF102578; AK031211; BC046384; BC052890 

Genbank Nucleotide ID

 AAF19055.1; BAC27301.1; AAH46384.1; AAH52890.1 

Protein Name

 Chloride intracellular channel protein 4 

Protein Synonyms/Alias

 mc3s5/mtCLIC 

Gene Name

 Clic4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
11	SMPLNGLKEEDKEPL	ubiquitination	[1]
103	EEVLCPPKYLKLSPK	ubiquitination	[1]
110	KYLKLSPKHPESNTA	ubiquitination	[1]
130	AKFSAYIKNSRPEAN	acetylation	[2, 3]
130	AKFSAYIKNSRPEAN	ubiquitination	[1]
146	ALERGLLKTLQKLDE	acetylation	[2]
146	ALERGLLKTLQKLDE	ubiquitination	[1]
150	GLLKTLQKLDEYLNS	ubiquitination	[1]
194	ADCNLLPKLHIVKVV	acetylation	[2]
194	ADCNLLPKLHIVKVV	ubiquitination	[1]
199	LPKLHIVKVVAKKYR	acetylation	[2]
199	LPKLHIVKVVAKKYR	ubiquitination	[1]
212	YRNFDIPKGMTGIWR	acetylation	[2]
212	YRNFDIPKGMTGIWR	ubiquitination	[1]
238	TNTCPSDKEVEIAYS	acetylation	[2]
238	TNTCPSDKEVEIAYS	ubiquitination	[1]
249	IAYSDVAKRLTK***	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3 (By similarity). May play a role in angiogenesis. 

Sequence Annotation

 DOMAIN 104 244 GST C-terminal.
 REGION 2 101 Required for insertion into the membrane
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 4 4 Phosphoserine (By similarity).
 MOD_RES 130 130 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Cell membrane; Chloride; Chloride channel; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Ion channel; Ion transport; Membrane; Mitochondrion; Nucleus; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 253 AA 

Protein Sequence

Gene Ontology

 GO:0045177; C:apical part of cell; IEA:Compara.
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
 GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005902; C:microvillus; IEA:Compara.
 GO:0030496; C:midbody; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0016363; C:nuclear matrix; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005254; F:chloride channel activity; ISO:MGI.
 GO:0005247; F:voltage-gated chloride channel activity; IEA:InterPro.
 GO:0001525; P:angiogenesis; IMP:MGI.
 GO:0048754; P:branching morphogenesis of an epithelial tube; IMP:MGI.
 GO:0071277; P:cellular response to calcium ion; IEA:Compara.
 GO:0001886; P:endothelial cell morphogenesis; IMP:MGI.
 GO:0009566; P:fertilization; IMP:MGI.
 GO:0030216; P:keratinocyte differentiation; IMP:UniProtKB.
 GO:0035264; P:multicellular organism growth; IMP:MGI.
 GO:0030336; P:negative regulation of cell migration; ISS:UniProtKB.
 GO:0061299; P:retina vasculature morphogenesis in camera-type eye; IMP:MGI.
 GO:0007035; P:vacuolar acidification; IMP:MGI. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR002946; Int_Cl_channel.
 IPR012336; Thioredoxin-like_fold. 

Pfam

  

SMART

  

PROSITE

 PS50405; GST_CTER 

PRINTS

 PR01263; INTCLCHANNEL.