CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014127
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Proteasome-associated protein ECM29 homolog 
Protein Synonyms/Alias
 Ecm29 
Gene Name
 ECM29 
Gene Synonyms/Alias
 KIAA0368 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46FLPPVLLKLSSTQEGubiquitination[1, 2]
244PEQLEQCKLGIVKFIubiquitination[2]
285TAADLELKSKQSLIDubiquitination[3]
287ADLELKSKQSLIDWNubiquitination[2]
301NNPAIINKMYKVYLGubiquitination[2]
313YLGDIPLKTKEGAVLubiquitination[1, 2, 3, 4]
315GDIPLKTKEGAVLKPubiquitination[2]
336VSTRVKLKIVPHLLRubiquitination[2]
410TKLINEYKEDPKLLSubiquitination[2]
414NEYKEDPKLLSMAYSubiquitination[1]
502QVRQVAVKFASTVFPubiquitination[1, 2, 5]
837SEGSGFTKLHLVESLubiquitination[3, 4]
852LSRIPSSKETNKMKEubiquitination[3]
856PSSKETNKMKERAIQubiquitination[3]
1039LMTGKRVKHEVSGETubiquitination[2]
1056FQGGALGKTPDGQGLubiquitination[2, 3, 4]
1099HAMWNSRKGAAFGFNubiquitination[2]
1324LRATEQEKAAMDSARubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
 Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis. 
Sequence Annotation
 REPEAT 34 71 HEAT 1.
 REPEAT 113 150 HEAT 2.
 REPEAT 168 211 HEAT 3.
 REPEAT 332 368 HEAT 4.
 REPEAT 393 432 HEAT 5.
 REPEAT 435 472 HEAT 6.
 REPEAT 475 513 HEAT 7.
 REPEAT 689 726 HEAT 8.
 REPEAT 727 765 HEAT 9.
 REPEAT 789 826 HEAT 10.
 REPEAT 835 874 HEAT 11.
 REPEAT 876 913 HEAT 12.
 REPEAT 937 975 HEAT 13.
 REPEAT 981 1018 HEAT 14.
 REPEAT 1019 1056 HEAT 15.
 REPEAT 1118 1155 HEAT 16.
 REPEAT 1158 1195 HEAT 17.
 REPEAT 1200 1237 HEAT 18.
 REPEAT 1249 1287 HEAT 19.
 REPEAT 1291 1329 HEAT 20.
 REPEAT 1354 1392 HEAT 21.
 REPEAT 1396 1433 HEAT 22.
 REPEAT 1523 1560 HEAT 23.
 REPEAT 1564 1601 HEAT 24.
 REPEAT 1611 1648 HEAT 25.
 REPEAT 1652 1689 HEAT 26.
 REPEAT 1785 1828 HEAT 27.
 MOD_RES 830 830 Phosphoserine.
 MOD_RES 836 836 Phosphothreonine.  
Keyword
 Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endoplasmic reticulum; Endosome; Nucleus; Phosphoprotein; Polymorphism; Proteasome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1845 AA 
Protein Sequence
MAAAAASASQ DELNQLERVF LRLGHAETDE QLQNIISKFL PPVLLKLSST QEGVRKKVME 60
LLVHLNKRIK SRPKIQLPVE TLLVQYQDPA AVSFVTNFTI IYVKMGYPRL PVEKQCELAP 120
TLLTAMEGKP QPQQDSLMHL LIPTLFHMKY PVESSKSASP FNLAEKPKTV QLLLDFMLDV 180
LLMPYGYVLN ESQSRQNSSS AQGSSSNSGG GSGIPQPPPG MSFYAAKRVI GDNPWTPEQL 240
EQCKLGIVKF IEAEQVPELE AVLHLVIASS DTRHSVATAA DLELKSKQSL IDWNNPAIIN 300
KMYKVYLGDI PLKTKEGAVL KPELKRDPVS TRVKLKIVPH LLRSRQAAET FPANIQVVYD 360
GLFGTNTNSK LRTLSLQFVH HICITCPEIK IKPLGPMLLN GLTKLINEYK EDPKLLSMAY 420
SAVGKLSSRM PHLFTKDIAL VQQLFEALCK EEPETRLAIQ EALSMMVGAY STLEGAQRTL 480
MEALVASYLI KPEVQVRQVA VKFASTVFPS DHIPSRYLLL LAAGDPREEV HGEAQRVLRC 540
LPGRNRKEST SEQMPSFPEM VYYIQEKASH RMKTPVKYMT GTTVLPFNPA AFGEIVLYLR 600
MCLAHSAGVV PTSQSLADMQ DHAPAIGRYI RTLMSSGQMA PSSSNKSGET NPVQIYIGLL 660
QQLLAGVGGL PVMYCLLEAV SVYPEKLATK FVDKTEWIKS LMNNSKEEMR ELAALFYSVV 720
VSTVSGNELK SMIEQLIKTT KDNHSPEIQH GSLLALGFTV GRYLAKKKMR MSEQQDLERN 780
ADTLPDQEEL IQSATETIGS FLDSTSPLLA IAACTALGEI GRNGPLPIPS EGSGFTKLHL 840
VESLLSRIPS SKETNKMKER AIQTLGYFPV GDGDFPHQKL LLQGLMDSVE AKQIELQFTI 900
GEAITSAAIG TSSVAARDAW QMTEEEYTPP AGAKVNDVVP WVLDVILNKH IISPNPHVRQ 960
AACIWLLSLV RKLSTHKEVK SHLKEIQSAF VSVLSENDEL SQDVASKGLG LVYELGNEQD 1020
QQELVSTLVE TLMTGKRVKH EVSGETVVFQ GGALGKTPDG QGLSTYKELC SLASDLSQPD 1080
LVYKFMNLAN HHAMWNSRKG AAFGFNVIAT RAGEQLAPFL PQLVPRLYRY QFDPNLGIRQ 1140
AMTSIWNALV TDKSMVDKYL KEILQDLVKN LTSNMWRVRE SSCLALNDLL RGRPLDDIID 1200
KLPEIWETLF RVQDDIKESV RKAAELALKT LSKVCVKMCD PAKGAAGQRT IAALLPCLLD 1260
KGMMSTVTEV RALSINTLVK ISKSAGAMLK PHAPKLIPAL LESLSVLEPQ VLNYLSLRAT 1320
EQEKAAMDSA RLSAAKSSPM METINMCLQY LDVSVLGELV PRLCELIRSG VGLGTKGGCA 1380
SVIVSLTTQC PQDLTPYSGK LMSALLSGLT DRNSVIQKSC AFAMGHLVRT SRDSSTEKLL 1440
QKLNGWYMEK EEPIYKTSCA LTIHAIGRYS PDVLKNHAKE VLPLAFLGMH EIADEEKSEK 1500
EECNLWTEVW QENVPGSFGG IRLYLQELIT ITQKALQSQS WKMKAQGAIA MASIAKQTSS 1560
LVPPYLGMIL TALLQGLAGR TWAGKEELLK AIACVVTACS AELEKSVPNQ PSTNEILQAV 1620
LKECSKENVK YKIVAISCAA DILKATKEDR FQEFSNIVIP LIKKNSLESS GVRTTKNEEE 1680
NEKEKELQLE YLLGAFESLG KAWPRNAETQ RCYRQELCKL MCERLKLSTW KVQLGVLQSM 1740
NAFFQGLMLL EEEHADPEAL AEILLETCKS ITYSLENKTY SSVRTEALSV IELLLKKLEE 1800
SKQWECLTSE CRVLLIESLA TMEPDSRPEL QEKAALLKKT LENLE 1845 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0030139; C:endocytic vesicle; IDA:UniProtKB.
 GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
 GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IEA:UniProtKB-SubCell.
 GO:0030134; C:ER to Golgi transport vesicle; IDA:UniProtKB.
 GO:0005771; C:multivesicular body; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0030433; P:ER-associated protein catabolic process; IMP:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR026827; ECM29/GCN1.
 IPR026826; ECM29_metazoa.
 IPR024372; Proteasome_stabil_ECM29. 
Pfam
 PF13001; Ecm29 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS