CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008903
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Adenylate kinase 2, mitochondrial 
Protein Synonyms/Alias
 AK 2; ATP-AMP transphosphorylase 2; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Adenylate kinase 2, mitochondrial, N-terminally processed 
Gene Name
 AK2 
Gene Synonyms/Alias
 ADK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14AAEPEYPKGIRAVLLacetylation[1]
28LGPPGAGKGTQAPRLubiquitination[2, 3]
93NLETPLCKNGFLLDGubiquitination[3, 4, 5, 6]
117MLDDLMEKRKEKLDSubiquitination[2, 3]
233AFSKATCKDLVMFI*ubiquitination[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis. 
Sequence Annotation
 NP_BIND 25 30 ATP.
 NP_BIND 72 74 AMP (By similarity).
 NP_BIND 100 103 AMP (By similarity).
 NP_BIND 151 152 ATP.
 REGION 45 74 NMPbind.
 REGION 141 178 LID.
 BINDING 46 46 AMP (By similarity).
 BINDING 51 51 AMP (By similarity).
 BINDING 107 107 AMP (By similarity).
 BINDING 138 138 ATP.
 BINDING 142 142 ATP (By similarity).
 BINDING 175 175 AMP.
 BINDING 186 186 AMP (By similarity).
 BINDING 214 214 ATP; via carbonyl oxygen.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 93 93 N6-acetyllysine (By similarity).
 DISULFID 42 92  
Keyword
 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Direct protein sequencing; Disease mutation; Disulfide bond; Kinase; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 239 AA 
Protein Sequence
MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL 60
GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE 120
KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE 180
KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI 239 
Gene Ontology
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005758; C:mitochondrial intermembrane space; TAS:Reactome.
 GO:0097226; C:sperm mitochondrial sheath; IEA:Compara.
 GO:0004017; F:adenylate kinase activity; TAS:ProtInc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006172; P:ADP biosynthetic process; IEA:Compara.
 GO:0046033; P:AMP metabolic process; IEA:Compara.
 GO:0046034; P:ATP metabolic process; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0046060; P:dATP metabolic process; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0006119; P:oxidative phosphorylation; IEA:Compara.
 GO:0097066; P:response to thyroid hormone stimulus; IEA:Compara. 
Interpro
 IPR006259; Adenyl_kin_sub.
 IPR000850; Adenylate_kin.
 IPR007862; Adenylate_kinase_lid-dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00406; ADK
 PF05191; ADK_lid 
SMART
  
PROSITE
 PS00113; ADENYLATE_KINASE 
PRINTS
 PR00094; ADENYLTKNASE.