UniProt Accession

 ASAH2_HUMAN; Q9NR71; Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6 

Genbank Protein ID

 AY049008; AF449759; AL450382; AL589794; AL589794; AL954360; AF250847; BC107105 

Genbank Nucleotide ID

 AAL06061.1; AAQ04667.2; CAI15870.1; CAI15766.1; CAI15767.1; CAI17190.1; AAF86240.1; AAI07106.1 

Protein Name

 Neutral ceramidase 

Protein Synonyms/Alias

 N-CDase; NCDase; Acylsphingosine deacylase 2; BCDase; LCDase; hCD; N-acylsphingosine amidohydrolase 2; Non-lysosomal ceramidase; Neutral ceramidase soluble form 

Gene Name

 ASAH2 

Gene Synonyms/Alias

 HNAC1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
663	GDVLQPAKPEYRVGE	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. 

Sequence Annotation

 REGION 770 780 Required for correct folding and
 ACT_SITE 354 354 Nucleophile.
 CARBOHYD 62 62 O-linked (GalNAc...) (Potential).
 CARBOHYD 67 67 O-linked (GalNAc...) (Potential).
 CARBOHYD 68 68 O-linked (GalNAc...) (Potential).
 CARBOHYD 70 70 O-linked (GalNAc...) (Potential).
 CARBOHYD 73 73 O-linked (GalNAc...) (Potential).
 CARBOHYD 74 74 O-linked (GalNAc...) (Potential).
 CARBOHYD 76 76 O-linked (GalNAc...) (Potential).
 CARBOHYD 78 78 O-linked (GalNAc...) (Potential).
 CARBOHYD 79 79 O-linked (GalNAc...) (Potential).
 CARBOHYD 80 80 O-linked (GalNAc...) (Potential).
 CARBOHYD 82 82 O-linked (GalNAc...) (Potential).
 CARBOHYD 84 84 O-linked (GalNAc...) (Potential).
 CARBOHYD 98 98 N-linked (GlcNAc...) (Potential).
 CARBOHYD 151 151 N-linked (GlcNAc...) (Potential).
 CARBOHYD 217 217 N-linked (GlcNAc...) (Potential).
 CARBOHYD 468 468 N-linked (GlcNAc...) (Potential).
 CARBOHYD 564 564 N-linked (GlcNAc...) (Potential).
 CARBOHYD 730 730 N-linked (GlcNAc...) (Potential).
 CARBOHYD 779 779 O-linked (GalNAc...) (Potential).  

Keyword

 Alternative splicing; Apoptosis; Cell membrane; Complete proteome; Glycoprotein; Hydrolase; Lipid metabolism; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Signal-anchor; Sphingolipid metabolism; Transmembrane; Transmembrane helix; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 780 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005739; C:mitochondrion; TAS:ProtInc.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0017040; F:ceramidase activity; IEA:EC.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006672; P:ceramide metabolic process; TAS:ProtInc.
 GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR006823; Ceramidase_alk. 

Pfam

 PF04734; Ceramidase_alk 

SMART

  

PROSITE

  

PRINTS