CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000998
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General vesicular transport factor p115 
Protein Synonyms/Alias
 Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein 
Gene Name
 USO1 
Gene Synonyms/Alias
 VDP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
25TEAETIQKLCDRVASubiquitination[1]
206RSNGAIQKIVAFENAubiquitination[1, 2]
637ELEGVITKAIYKSSEubiquitination[1]
654KKEEEVKKTLEQHDNubiquitination[1]
716KDQYNLLKIQLGKDNubiquitination[1]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity). 
Sequence Annotation
 REPEAT 20 60 ARM 1.
 REPEAT 61 121 ARM 2.
 REPEAT 123 163 ARM 3.
 REPEAT 166 207 ARM 4.
 REPEAT 208 253 ARM 5.
 REPEAT 255 310 ARM 6.
 REPEAT 311 354 ARM 7.
 REPEAT 363 408 ARM 8.
 REPEAT 420 459 ARM 9.
 REPEAT 473 513 ARM 10.
 REPEAT 518 571 ARM 11.
 REPEAT 573 630 ARM 12.
 REGION 1 637 Globular head.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 202 202 N6-acetyllysine.
 MOD_RES 942 942 Phosphoserine.
 MOD_RES 952 952 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 962 AA 
Protein Sequence
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 60
AMEHLIHVLQ TDRSDSEIIG YALDTLYNII SNEEEEEVDD VEEENSTRQS EDLGSQFTEI 120
FIKQQENVTL LLSLLEEFDF HVRWPGVKLL TSLLKQLGPQ VQQIILVSPM GVSRLMDLLA 180
DSREVIRNDG VLLLQALTRS NGAIQKIVAF ENAFERLLDI ISEEGNSDGG IVVEDCLILL 240
QNLLKNNNSN QNFFKEGSYI QRMKPWFEVG DENSGWSAQK VTNLHLMLQL VRVLVSPTNP 300
PGATSSCQKA MFQCGLLQQL CTILMATGVP ADILTETINT VSEVIRGCQV NQDYFASVNA 360
PSNPPRPAIV VLLMSMVNER QPFVLRCAVL YCFQCFLYKN QKGQGEIVST LLPSTIDATG 420
NSVSAGQLLC GGLFSTDSLS NWCAAVALAH ALQENATQKE QLLRVQLATS IGNPPVSLLQ 480
QCTNILSQGD KIDRRGSKIQ TRVGLLMLLC TWLSNCPIAV THFLHNSANV PFLTGQIAEN 540
LGEEEQLVQG LCALLLGISI YFNDNSLESY MKEKLKQLIE KRIGKENFIE KLGFISKHEL 600
YSRASQKPQP NFPSPEYMIF DHEFTKLVKE LEGVITKAIY KSSEEDKKEE EVKKTLEQHD 660
NIVTHYKNMI REQDLQLEEL RQQVSTLKCQ NEQLQTAVTQ QVSQIQQHKD QYNLLKIQLG 720
KDNQHQGSYS EGAQMNGIQP EEIGRLREEI EELKRNQELL QSQLTEKDSM IENMKSSQTS 780
GTNEQSSAIV SARDSEQVAE LKQELATLKS QLNSQSVEIT KLQTEKQELL QKTEAFAKSV 840
EVQGETETII ATKTTDVEGR LSALLQETKE LKNEIKALSE ERTAIKEQLD SSNSTIAILQ 900
TEKDKLELEI TDSKKEQDDL LVLLADQDQK ILSLKNKLKD LGHPVEEEDE LESGDQEDED 960
DESEDPGKDL DHI 973 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:ProtInc.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR006955; Uso1_p115_C.
 IPR024095; Vesicle_P115-like.
 IPR006953; Vesicle_Uso1_P115_head. 
Pfam
 PF04871; Uso1_p115_C
 PF04869; Uso1_p115_head 
SMART
  
PROSITE
 PS50176; ARM_REPEAT 
PRINTS