CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004073
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase 
Protein Synonyms/Alias
 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase 
Gene Name
 bioA 
Gene Synonyms/Alias
 b0774; JW0757 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
229MYHPEWLKRIRKICDacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor. 
Sequence Annotation
 REGION 112 113 Pyridoxal phosphate binding.
 REGION 308 309 Pyridoxal phosphate binding.
 BINDING 52 52 Substrate.
 BINDING 144 144 Substrate (Probable).
 BINDING 245 245 Pyridoxal phosphate.
 BINDING 274 274 Substrate.
 BINDING 307 307 Substrate; via carbonyl oxygen.
 BINDING 391 391 Substrate.
 MOD_RES 274 274 N6-(pyridoxal phosphate)lysine.  
Keyword
 3D-structure; Aminotransferase; Biotin biosynthesis; Complete proteome; Cytoplasm; Pyridoxal phosphate; Reference proteome; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 429 AA 
Protein Sequence
MTTDDLAFDQ RHIWHPYTSM TSPLPVYPVV SAEGCELILS DGRRLVDGMS SWWAAIHGYN 60
HPQLNAAMKS QIDAMSHVMF GGITHAPAIE LCRKLVAMTP QPLECVFLAD SGSVAVEVAM 120
KMALQYWQAK GEARQRFLTF RNGYHGDTFG AMSVCDPDNS MHSLWKGYLP ENLFAPAPQS 180
RMDGEWDERD MVGFARLMAA HRHEIAAVII EPIVQGAGGM RMYHPEWLKR IRKICDREGI 240
LLIADEIATG FGRTGKLFAC EHAEIAPDIL CLGKALTGGT MTLSATLTTR EVAETISNGE 300
AGCFMHGPTF MGNPLACAAA NASLAILESG DWQQQVADIE VQLREQLAPA RDAEMVADVR 360
VLGAIGVVET THPVNMAALQ KFFVEQGVWI RPFGKLIYLM PPYIILPQQL QRLTAAVNRA 420
VQDETFFCQ 429 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004015; F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity; IDA:UniProtKB.
 GO:0030170; F:pyridoxal phosphate binding; IDA:UniProtKB.
 GO:0009102; P:biotin biosynthetic process; IDA:UniProtKB. 
Interpro
 IPR005814; Aminotrans_3.
 IPR005815; BioA.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 
Pfam
 PF00202; Aminotran_3 
SMART
  
PROSITE
 PS00600; AA_TRANSFER_CLASS_3 
PRINTS