CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019379
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromosome alignment-maintaining phosphoprotein 1 
Protein Synonyms/Alias
 Zinc finger protein 828 
Gene Name
 CHAMP1 
Gene Synonyms/Alias
 C13orf8; CAMP; KIAA1802; ZNF828 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66AKLFHCHKCFFTSKMubiquitination[1, 2, 3]
349SVSPGPWKPIPSVSPacetylation[4]
490SFFIEPQKPVFPETRacetylation[4, 5, 6, 7]
490SFFIEPQKPVFPETRubiquitination[8]
516KAASDIWKPVLSIDTacetylation[4, 6]
617DTLFPSSKKLKKDNQacetylation[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore. 
Sequence Annotation
 ZN_FING 738 760 C2H2-type.
 REGION 271 490 Mediates interaction with MAD2L2.
 REGION 451 590 Mediates localization to the spindle and
 REGION 591 812 Mediates localization to the chromosome
 MOD_RES 87 87 Phosphoserine.
 MOD_RES 108 108 Phosphoserine.
 MOD_RES 184 184 Phosphoserine.
 MOD_RES 204 204 Phosphoserine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 217 217 Phosphoserine.
 MOD_RES 247 247 Phosphoserine.
 MOD_RES 253 253 Phosphoserine.
 MOD_RES 275 275 Phosphoserine.
 MOD_RES 282 282 Phosphoserine.
 MOD_RES 286 286 Phosphoserine.
 MOD_RES 297 297 Phosphoserine.
 MOD_RES 308 308 Phosphoserine.
 MOD_RES 319 319 Phosphoserine.
 MOD_RES 344 344 Phosphoserine.
 MOD_RES 355 355 Phosphoserine.
 MOD_RES 382 382 Phosphoserine.
 MOD_RES 386 386 Phosphoserine.
 MOD_RES 405 405 Phosphoserine.
 MOD_RES 416 416 Phosphoserine.
 MOD_RES 427 427 Phosphoserine.
 MOD_RES 432 432 Phosphoserine.
 MOD_RES 436 436 Phosphoserine.
 MOD_RES 443 443 Phosphoserine.
 MOD_RES 445 445 Phosphoserine.
 MOD_RES 452 452 Phosphoserine.
 MOD_RES 459 459 Phosphoserine.
 MOD_RES 462 462 Phosphoserine.
 MOD_RES 476 476 Phosphoserine.
 MOD_RES 490 490 N6-acetyllysine.
 MOD_RES 507 507 Phosphoserine.
 MOD_RES 542 542 Phosphoserine.
 MOD_RES 603 603 Phosphoserine.
 MOD_RES 626 626 Phosphoserine.
 MOD_RES 627 627 Phosphoserine.
 MOD_RES 632 632 Phosphoserine.
 MOD_RES 651 651 Phosphoserine.
 MOD_RES 652 652 Phosphoserine.
 MOD_RES 653 653 Phosphoserine.
 MOD_RES 675 675 Phosphoserine.
 MOD_RES 736 736 Phosphoserine.  
Keyword
 Acetylation; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 812 AA 
Protein Sequence
MEAFQELRKP SARLECDHCS FRGTDYENVQ IHMGTIHPEF CDEMDAGGLG KMIFYQKSAK 60
LFHCHKCFFT SKMYSNVYYH ITSKHASPDK WNDKPKNQLN KETDPVKSPP LPEHQKIPCN 120
SAEPKSIPAL SMETQKLGSV LSPESPKPTP LTPLEPQKPG SVVSPELQTP LPSPEPSKPA 180
SVSSPEPPKS VPVCESQKLA PVPSPEPQKP APVSPESVKA TLSNPKPQKQ SHFPETLGPP 240
SASSPESPVL AASPEPWGPS PAASPESRKS ARTTSPEPRK PSPSESPEPW KPFPAVSPEP 300
RRPAPAVSPG SWKPGPPGSP RPWKSNPSAS SGPWKPAKPA PSVSPGPWKP IPSVSPGPWK 360
PTPSVSSASW KSSSVSPSSW KSPPASPESW KSGPPELRKT APTLSPEHWK AVPPVSPELR 420
KPGPPLSPEI RSPAGSPELR KPSGSPDLWK LSPDQRKTSP ASLDFPESQK SSRGGSPDLW 480
KSSFFIEPQK PVFPETRKPG PSGPSESPKA ASDIWKPVLS IDTEPRKPAL FPEPAKTAPP 540
ASPEARKRAL FPEPRKHALF PELPKSALFS ESQKAVELGD ELQIDAIDDQ KCDILVQEEL 600
LASPKKLLED TLFPSSKKLK KDNQESSDAE LSSSEYIKTD LDAMDIKGQE SSSDQEQVDV 660
ESIDFSKENK MDMTSPEQSR NVLQFTEEKE AFISEEEIAK YMKRGKGKYY CKICCCRAMK 720
KGAVLHHLVN KHNVHSPYKC TICGKAFLLE SLLKNHVAAH GQSLLKCPRC NFESNFPRGF 780
KKHLTHCQSR HNEEANKKLM EALEPPLEEQ QI 812 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0034501; P:protein localization to kinetochore; IMP:UniProtKB.
 GO:0035372; P:protein localization to microtubule; IMP:UniProtKB.
 GO:0031134; P:sister chromatid biorientation; IMP:UniProtKB. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS