CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016991
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Coiled-coil domain-containing protein 50 
Protein Synonyms/Alias
 Protein Ymer 
Gene Name
 CCDC50 
Gene Synonyms/Alias
 C3orf6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
60QHDLQVAKQLQEEDLubiquitination[1, 2, 3]
68QLQEEDLKAQAQLQKubiquitination[1]
94IAQEIQEKLAIEAERubiquitination[3, 4]
108RRRIQEKKDEDIARLubiquitination[1, 3, 4]
119IARLLQEKELQEEKKubiquitination[1, 3, 4, 5, 6]
128LQEEKKRKKHFPEFPubiquitination[4, 5, 6]
129QEEKKRKKHFPEFPAubiquitination[1, 3, 4, 5, 6, 7, 8, 9]
152YYEDGGMKPRVMKEAubiquitination[1, 3, 4, 5, 6, 8, 9, 10, 11]
157GMKPRVMKEAVSTPSubiquitination[1, 3, 4, 9, 10, 11, 12]
181YDAEIARKLQEEELLubiquitination[1, 2, 3, 4, 5, 6]
214RLLMAEEKKAYKKAKubiquitination[1, 3, 4]
225KKAKEREKSSLDKRKubiquitination[1, 3]
232KSSLDKRKQDPEWKPubiquitination[1, 3, 4, 5, 6]
238RKQDPEWKPKTAKAAubiquitination[1, 3, 4]
240QDPEWKPKTAKAANSubiquitination[3, 4]
250KAANSKSKESDEPHHubiquitination[1]
259SDEPHHSKNERPARPubiquitination[3, 4]
294SSTRHFSKSESSHKGubiquitination[3]
300SKSESSHKGFHYKH*ubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [8] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [9] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [10] A data set of human endogenous protein ubiquitination sites.
 Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
 Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
 [11] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [12] Proteomic snapshot of the EGF-induced ubiquitin network.
 Argenzio E, Bange T, Oldrini B, Bianchi F, Peesari R, Mari S, Di Fiore PP, Mann M, Polo S.
 Mol Syst Biol. 2011 Jan 18;7:462. [PMID: 21245847
Functional Description
 Involved in EGFR signaling. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; Deafness; Non-syndromic deafness; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 306 AA 
Protein Sequence
MAEVSIDQSK LPGVKEVCRD FAVLEDHTLA HSLQEQEIEH HLASNVQRNR LVQHDLQVAK 60
QLQEEDLKAQ AQLQKRYKDL EQQDCEIAQE IQEKLAIEAE RRRIQEKKDE DIARLLQEKE 120
LQEEKKRKKH FPEFPATRAY ADSYYYEDGG MKPRVMKEAV STPSRMAHRD QEWYDAEIAR 180
KLQEEELLAT QVDMRAAQVA QDEEIARLLM AEEKKAYKKA KEREKSSLDK RKQDPEWKPK 240
TAKAANSKSK ESDEPHHSKN ERPARPPPPI MTDGEDADYT HFTNQQSSTR HFSKSESSHK 300
GFHYKH 306 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA. 
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS