UniProt Accession

 CALR_RAT; P18418; P10452 

Genbank Protein ID

 X53363; D78308; X79327; BC062395; X13702 

Genbank Nucleotide ID

 CAA37446.1; BAA11345.1; CAA55890.1; AAH62395.1; CAA31987.1 

Protein Name

 Calreticulin 

Protein Synonyms/Alias

 CALBP; CRP55; Calcium-binding protein 3; CABP3; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP 

Gene Name

 Calr 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
41	TNRWVESKHKSDFGK	acetylation	[1]
48	KHKSDFGKFVLSSGK	acetylation	[1]
55	KFVLSSGKFYGDQEK	acetylation	[1]
62	KFYGDQEKDKGLQTS	acetylation	[1]
151	VHVIFNYKGKNVLIN	acetylation	[1]
159	GKNVLINKDIRCKDD	acetylation	[1]
206	DWDFLPPKKIKDPDA	acetylation	[1]
209	FLPPKKIKDPDAAKP	acetylation	[1]
215	IKDPDAAKPEDWDER	acetylation	[1]
232	IDDPTDSKPEDWDKP	acetylation	[1]
238	SKPEDWDKPEHIPDP	acetylation	[1]
368	QDEEQRLKEEEEDKK	acetylation	[1]
374	LKEEEEDKKRKEEEE	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). 

Sequence Annotation

 REPEAT 191 202 1-1.
 REPEAT 210 221 1-2.
 REPEAT 227 238 1-3.
 REPEAT 244 255 1-4.
 REPEAT 259 269 2-1.
 REPEAT 273 283 2-2.
 REPEAT 287 297 2-3.
 REGION 18 197 N-domain.
 REGION 191 255 4 X approximate repeats.
 REGION 198 308 P-domain.
 REGION 259 297 3 X approximate repeats.
 REGION 309 416 C-domain.
 MOTIF 413 416 Prevents secretion from ER.
 METAL 26 26 Calcium; via carbonyl oxygen (By
 METAL 62 62 Calcium; via carbonyl oxygen (By
 METAL 64 64 Calcium; via carbonyl oxygen (By
 METAL 328 328 Calcium (By similarity).
 BINDING 109 109 Carbohydrate (By similarity).
 BINDING 111 111 Carbohydrate (By similarity).
 BINDING 128 128 Carbohydrate (By similarity).
 BINDING 135 135 Carbohydrate (By similarity).
 BINDING 317 317 Carbohydrate (By similarity).
 MOD_RES 48 48 N6-acetyllysine (By similarity).
 MOD_RES 159 159 N6-acetyllysine (By similarity).
 MOD_RES 209 209 N6-acetyllysine (By similarity).
 DISULFID 105 137 By similarity.  

Keyword

 3D-structure; Acetylation; Calcium; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Lectin; Metal-binding; Reference proteome; Repeat; Sarcoplasmic reticulum; Signal; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 416 AA 

Protein Sequence

Gene Ontology

 GO:0001669; C:acrosomal vesicle; IDA:RGD.
 GO:0009986; C:cell surface; IDA:RGD.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0031012; C:extracellular matrix; IDA:RGD.
 GO:0005615; C:extracellular space; IDA:RGD.
 GO:0005794; C:Golgi apparatus; IDA:RGD.
 GO:0042824; C:MHC class I peptide loading complex; IEA:Compara.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
 GO:0005844; C:polysome; IEA:Compara.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0016529; C:sarcoplasmic reticulum; IDA:RGD.
 GO:0033018; C:sarcoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IDA:RGD.
 GO:0030246; F:carbohydrate binding; IEA:Compara.
 GO:0042562; F:hormone binding; IPI:RGD.
 GO:0005506; F:iron ion binding; IDA:RGD.
 GO:0003729; F:mRNA binding; IDA:BHF-UCL.
 GO:0042277; F:peptide binding; IDA:RGD.
 GO:0051082; F:unfolded protein binding; IMP:RGD.
 GO:0055007; P:cardiac muscle cell differentiation; IEP:RGD.
 GO:0007050; P:cell cycle arrest; IEA:Compara.
 GO:0071285; P:cellular response to lithium ion; IEP:RGD.
 GO:0071310; P:cellular response to organic substance; IEP:RGD.
 GO:0090398; P:cellular senescence; IEA:Compara.
 GO:0061077; P:chaperone-mediated protein folding; IMP:RGD.
 GO:0030866; P:cortical actin cytoskeleton organization; IEA:Compara.
 GO:0033144; P:negative regulation of intracellular steroid hormone receptor signaling pathway; IEA:Compara.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:0048387; P:negative regulation of retinoic acid receptor signaling pathway; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0017148; P:negative regulation of translation; IDA:BHF-UCL.
 GO:0002502; P:peptide antigen assembly with MHC class I protein complex; IEA:Compara.
 GO:0045787; P:positive regulation of cell cycle; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:2000510; P:positive regulation of dendritic cell chemotaxis; IEA:Compara.
 GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0050766; P:positive regulation of phagocytosis; IEA:Compara.
 GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; IEA:Compara.
 GO:0006611; P:protein export from nucleus; IEA:Compara.
 GO:0034504; P:protein localization to nucleus; IEA:Compara.
 GO:0050821; P:protein stabilization; ISS:UniProtKB.
 GO:0040020; P:regulation of meiosis; IEA:Compara.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0032355; P:response to estradiol stimulus; IEP:RGD.
 GO:0033574; P:response to testosterone stimulus; IEP:RGD.
 GO:0007283; P:spermatogenesis; IEP:RGD. 

Interpro

 IPR001580; Calret/calnex.
 IPR018124; Calret/calnex_CS.
 IPR009169; Calreticulin.
 IPR008985; ConA-like_lec_gl_sf.
 IPR013320; ConA-like_subgrp. 

Pfam

 PF00262; Calreticulin 

SMART

  

PROSITE

 PS00803; CALRETICULIN_1
 PS00804; CALRETICULIN_2
 PS00805; CALRETICULIN_REPEAT
 PS00014; ER_TARGET 

PRINTS

 PR00626; CALRETICULIN.