CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039945
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 RAS guanyl-releasing protein 1 
Protein Synonyms/Alias
  
Gene Name
 RASGRP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
262HSSISRLKETSSHVPubiquitination[1]
274HVPHEINKVLGEMTEubiquitination[1]
399APPLTPSKPPVVVDWubiquitination[1]
432RMVDSVFKNYDHDQDubiquitination[1]
463FSFCVMDKDREGLISubiquitination[1]
536MNCHKQCKDLVVFECubiquitination[1]
545LVVFECKKRAKNPVAubiquitination[1]
548FECKKRAKNPVAPTEubiquitination[1]
649FVLSSPRKTAQDTLYubiquitination[1, 2]
682AFVKWENKDSLIKSKubiquitination[1]
727QLKYAQKKIESLQLEubiquitination[1, 2]
735IESLQLEKSNHVLAQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Calcium; Complete proteome; Guanine-nucleotide releasing factor; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 749 AA 
Protein Sequence
MMVSLGHLAK GASLDDLIDS CIQSFDADGN LCRSNQLLQV MLTMHRIVIS SAELLQKVIT 60
LYKDALAKNS PGLCLKICYF VRYWITEFWV MFKMDASLTD TMEEFQELVK AKGEELHCRL 120
IDTTQINARD WSRKLTQRIK SNTSKKRKVS LLFDHLEPEE LSEHLTYLEF KSFRRISFSD 180
YQNYLVNSCV KENPTMERSI ALCNGISQWV QLMVLSRPTP QLRAEVFIKF IQVAQKLHQL 240
QNFNTLMAVI GGLCHSSISR LKETSSHVPH EINKVLGEMT ELLSSSRNYD NYRRAYGECT 300
DFKIPILGVH LKDLISLYEA MPDYLEDGKV NVHKLLALYN HISELVQLQE VAPPLEANKD 360
LVHLLTLSLD LYYTEDEIYE LSYAREPRNH RAPPLTPSKP PVVVDWASGV SPKPDPKTIS 420
KHVQRMVDSV FKNYDHDQDG YISQEEFEKI AASFPFSFCV MDKDREGLIS RDEITAYFMR 480
ASSIYSKLGL GFPHNFQETT YLKPTFCDNC AGFLWGVIKQ GYRCKDCGMN CHKQCKDLVV 540
FECKKRAKNP VAPTENNTSV GPVSNLCSLG AKDLLHAPEE GPFTFPNGEA VEHGEESKDR 600
TIMLMGVSSQ KISLRLKRAV AHKATQTESQ PWIGSEGPSG PFVLSSPRKT AQDTLYVLPS 660
PTSPCPSPVL VRKRAFVKWE NKDSLIKSKE ELRHLRLPTY QELEQEINTL KADNDALKIQ 720
LKYAQKKIES LQLEKSNHVL AQMEQGDCS 749 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR020454; DAG/PE-bd.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000651; Ras-like_Gua-exchang_fac_N.
 IPR008937; Ras_GEF.
 IPR023578; Ras_GEF_dom.
 IPR001895; RasGRF_CDC25. 
Pfam
 PF00130; C1_1
 PF13405; EF_hand_4
 PF00617; RasGEF
 PF00618; RasGEF_N 
SMART
 SM00109; C1
 SM00147; RasGEF
 SM00229; RasGEFN 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50009; RASGEF_CAT
 PS50212; RASGEF_NTER
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS
 PR00008; DAGPEDOMAIN.