UniProt Accession

 NPC2_MOUSE; Q9Z0J0; Q3UB23 

Genbank Protein ID

 AB021289; AK008603; AK009127; AK151067; AK151133; AK153020; AK153203; AK158624; AK165263; AK167869; AK170327; BC003471; BC007190 

Genbank Nucleotide ID

 BAA35183.1; BAB25771.1; BAB26090.1; BAE30083.1; BAE30141.1; BAE31654.1; BAE31803.1; BAE34585.1; BAE38111.1; BAE39885.1; BAE41721.1; AAH03471.1; AAH07190.1 

Protein Name

 Epididymal secretory protein E1 

Protein Synonyms/Alias

 mE1; Niemann Pick type C2 protein homolog 

Gene Name

 Npc2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
30	HFKDCGSKVGVIKEV	acetylation	[1, 2]
30	HFKDCGSKVGVIKEV	succinylation	[2]
35	GSKVGVIKEVNVSPC	acetylation	[1]
94	IPEPDGCKSGINCPI	acetylation	[1]
103	GINCPIQKDKVYSYL	acetylation	[2]
103	GINCPIQKDKVYSYL	succinylation	[2]
105	NCPIQKDKVYSYLNK	acetylation	[1, 2]
105	NCPIQKDKVYSYLNK	succinylation	[2]
112	KVYSYLNKLPVKNEY	acetylation	[1]
116	YLNKLPVKNEYPSIK	acetylation	[1, 2]
129	IKLVVEWKLEDDKKN	acetylation	[1]
134	EWKLEDDKKNNLFCW	acetylation	[1]
135	WKLEDDKKNNLFCWE	acetylation	[1]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport. 

Sequence Annotation

 CARBOHYD 58 58 N-linked (GlcNAc...) (Potential).
 CARBOHYD 69 69 N-linked (GlcNAc...) (Potential).
 DISULFID 27 140 By similarity.
 DISULFID 42 47 By similarity.
 DISULFID 93 99 By similarity.  

Keyword

 Cholesterol metabolism; Complete proteome; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Lipid metabolism; Lysosome; Reference proteome; Secreted; Signal; Steroid metabolism; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 149 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0005764; C:lysosome; ISS:HGNC.
 GO:0015485; F:cholesterol binding; IMP:UniProtKB.
 GO:0033344; P:cholesterol efflux; IMP:UniProtKB.
 GO:0042632; P:cholesterol homeostasis; ISS:HGNC.
 GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
 GO:0032367; P:intracellular cholesterol transport; IMP:HGNC.
 GO:0009615; P:response to virus; IEA:Compara. 

Interpro

 IPR014756; Ig_E-set.
 IPR003172; MD-2_lipid-recog_dom. 

Pfam

 PF02221; E1_DerP2_DerF2 

SMART

 SM00737; ML 

PROSITE

  

PRINTS