CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031562
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD40 repeat-containing protein SMU1 
Protein Synonyms/Alias
 cDNA FLJ54259, highly similar to Smu-1 suppressor of mec-8 and unc-52 protein homolog 
Gene Name
 SMU1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33FRGKAAVKDVEEEKFubiquitination[1, 2, 3, 4]
39VKDVEEEKFPTQLSRubiquitination[1, 3, 5]
49TQLSRHIKFGQKSHVacetylation[4]
53RHIKFGQKSHVECARubiquitination[3, 4]
126ATGAQDGKIKVWKIQubiquitination[1, 3, 4, 6]
128GAQDGKIKVWKIQSGubiquitination[3]
131DGKIKVWKIQSGQCLubiquitination[3]
182LKSGKTLKEFRGHSSubiquitination[3]
218TVKIWNMKTTECSNTubiquitination[3, 4, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 352 AA 
Protein Sequence
MALLGQALKW QQHQGLLPPG MTIDLFRGKA AVKDVEEEKF PTQLSRHIKF GQKSHVECAR 60
FSPDGQYLVT GSVDGFIEVW NFTTGKIRKD LKYQAQDNFM MMDDAVLCMC FSRDTEMLAT 120
GAQDGKIKVW KIQSGQCLRR FERAHSKGVT CLSFSKDSSQ ILSASFDQTI RIHGLKSGKT 180
LKEFRGHSSF VNEATFTQDG HYIISASSDG TVKIWNMKTT ECSNTFKSLG STAGTDITVN 240
SVILLPKNPE HFVVCNRSNT VVIMNMQGQI VRSFSSGKRE GGDFVCCALS PRGEWIYCVG 300
EDFVLYCFST VTGKLERTLT VHEKDVIGIA HHPHQNLIAT YSEDGLLKLW KP 352 
Gene Ontology
  
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.