CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004542
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GTPase-activating protein SAC7 
Protein Synonyms/Alias
  
Gene Name
 SAC7 
Gene Synonyms/Alias
 YDR389W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
266KTNNVTVKSSRQNYNubiquitination[1]
284ANDGDIEKEDAKDDEubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 GTPase-activating protein for RHO1. Must be involved in the normal assembly or function or both of actin. Plays an essential role only at low temperatures. 
Sequence Annotation
 DOMAIN 134 393 Rho-GAP.
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 46 46 Phosphoserine.
 MOD_RES 435 435 Phosphoserine.
 MOD_RES 491 491 Phosphoserine.
 MOD_RES 632 632 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Cytoskeleton; GTPase activation; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 654 AA 
Protein Sequence
MPNNTLKQGS KIENVSPSKG HVPSFWKQFI NNPKSMSSEN ITVPRSPTSL SRNAQPTTLK 60
RPPLSSRPYS YNTPTKDRKS FSKSAKQNNN NNNANSGTSP HAEFKNYRDM FLSNRNGFTG 120
RVFGVTLAES LSVASAEVIV QSELVSFGRI PIVVAKCGAY LKANGLETSG IFRIAGNGKR 180
VKALQYIFSS PPDYGTKFND WETYTVHDVA SLLRRYLNNL AEPLIPLSLY EQFRNPLRSR 240
PRILRHMLTH EVSHPNANKT NNVTVKSSRQ NYNDDGANDG DIEKEDAKDD EEKRRRKIRH 300
KRRLTRDIRA AIKEYEELFV TLSNDTKQLT IYLLDLLSLF ARQSQFNLMS GRNLAAIFQP 360
SILSHPQHDM DPKEYELSRL VVEFLIEYSY KLLPHLLKLA KREQQERLST ENKKNNGDKQ 420
KTDPIEIPKI TSSDSPPIVS SNKNPPAIDN NNKLDHTTLS PISTSIPENS SDLQTSKMLK 480
PPKQRRPHSK SFGSTPVPPD VIASNKRRTS LFPWLHKPGI LSDTGDNGDL TATEAEGDDY 540
EEENVDPYGQ SPSSVHSGSL PKQHYLPIPR MNRSLSGNST NSSFNTRPIS MILTSGNDNS 600
ADQLELLSNT HSNNERSNAL PLTEDDGDER NSRSRKRESW FQRLTSRSGS ANRA 654 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; TAS:SGD.
 GO:0005100; F:Rho GTPase activator activity; TAS:SGD.
 GO:0030037; P:actin filament reorganization involved in cell cycle; IMP:SGD.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:SGD. 
Interpro
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00620; RhoGAP 
SMART
 SM00324; RhoGAP 
PROSITE
 PS50238; RHOGAP 
PRINTS