CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014511
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-Id 
Protein Synonyms/Alias
 Myosin heavy chain myr 4 
Gene Name
 Myo1d 
Gene Synonyms/Alias
 Myr4 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
238IRVGAQLKSSINDAAubiquitination[1]
260AMKVIGFKPEEIQTVacetylation[2]
478FLEALNSKLGKHGHFacetylation[2]
524IGFIDKNKDTLFQDFacetylation[2]
551KNMWPEGKLSITEVTubiquitination[1]
634AFRQTYEKFLHRYKMacetylation[2]
660PSDKEAVKKLIERCGacetylation[2]
886VTDRHLYKMDPTKQYacetylation[2]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). 
Sequence Annotation
 DOMAIN 2 682 Myosin head-like.
 DOMAIN 698 727 IQ 1.
 DOMAIN 728 741 IQ 2.
 NP_BIND 102 109 ATP (Potential).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 200 200 Phosphoserine (By similarity).
 MOD_RES 536 536 Phosphotyrosine (By similarity).  
Keyword
 Acetylation; Actin-binding; ATP-binding; Calmodulin-binding; Complete proteome; Motor protein; Myosin; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1006 AA 
Protein Sequence
MAEQESLEFG KADFVLMDTV SMPEFMANLR LRFEKGRIYT FIGEVVVSVN PYKVLNIYGR 60
DTIEQYKGRE LYERPPHLFA IADAAYKAMK RRSKDTCIMI SGESGAGKTE ASKYIMQYIA 120
AITNPSQRAE IERVKNMLLK SNCVLEAFGN AKTNRNDNSS RFGKYMDINF DFKGDPIGGH 180
INNYLLEKSR VIVQQPGERS FHSFYQLLQG GSEQMLHSLH LQKSLSSYNY IRVGAQLKSS 240
INDAAEFKVV ADAMKVIGFK PEEIQTVYKI LAAILHLGNL KFIVDGDTPL IENGKVVSVI 300
AELLSTKADM VEKALLYRTV ATGRDIIDKQ HTEQEASYGR DAFAKAIYER LFCWIVTRIN 360
DIIEVKNYDT TVHGKNTVIG VLDIYGFEIF DNNSFEQFCI NYCNEKLQQL FIQLVLKQEQ 420
EEYQREGIPW KHIDYFNNQI IVDLVEQQHK GIIAILDDAC MNVGKVTDGM FLEALNSKLG 480
KHGHFSSRKT CASDKILEFD RDFRIRHYAG DVVYSVIGFI DKNKDTLFQD FKRLMYNSSN 540
PVLKNMWPEG KLSITEVTKR PLTAATLFKN SMIALVDNLA SKEPYYVRCI KPNDKKSPQI 600
FDDERCRHQV EYLGLLENVR VRRAGFAFRQ TYEKFLHRYK MISEFTWPNH DLPSDKEAVK 660
KLIERCGFQD DVAYGKTKIF IRTPRTLFTL EELRAQMLVR VVLFLQKVWR GTLARMRYKR 720
TKAALTIIRY YRRYKVKSYI HEVARRFHGV KNMRDYGKHV KWPTPPKVLR RFEEALQSIF 780
NRWRASQLIK TIPASDLPQV RAKVAAMEML KGQRADLGLQ RAWEGNYLAS KPDTPQTSGT 840
FVPVANELKR KDKYMNVLFS CHVRKVNRFS KVEDRAIFVT DRHLYKMDPT KQYKVMKTIP 900
LYNLTGLSVS NGKDQLVVFH TKDNKDLIVC LFSKQPTHES RIGELVGVLV NHFKSEKRHL 960
QVNVTNPVQC SLHGKKCTVS VETRLNQPQP DFTKNRSGFI LSVPGN 1006 
Gene Ontology
 GO:0030673; C:axolemma; IDA:RGD.
 GO:0016459; C:myosin complex; IDA:RGD.
 GO:0005790; C:smooth endoplasmic reticulum; IDA:RGD.
 GO:0030898; F:actin-dependent ATPase activity; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0048306; F:calcium-dependent protein binding; IDA:RGD.
 GO:0005516; F:calmodulin binding; IDA:RGD.
 GO:0000146; F:microfilament motor activity; IDA:RGD.
 GO:0010923; P:negative regulation of phosphatase activity; ISS:UniProtKB. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR010926; Myosin_tail_2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00612; IQ
 PF00063; Myosin_head
 PF06017; Myosin_TH1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.