UniProt Accession

 UBC9_RAT; P63281; P50550; Q15698; Q86VB3 

Genbank Protein ID

 U54632; BC086324; BC086592 

Genbank Nucleotide ID

 AAC98704.1; AAH86324.1; AAH86592.1 

Protein Name

 SUMO-conjugating enzyme UBC9 

Protein Synonyms/Alias

 SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-conjugating enzyme UbcE2A; Ubiquitin-protein ligase I 

Gene Name

 Ube2i 

Gene Synonyms/Alias

 Ubce9 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
65	FKLRMLFKDDYPSSP	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Essential for nuclear architecture and chromosome segregation (By similarity). Necessary for sumoylation of FOXL2 and KAT5. Sumoylates p53/TP53 at 'Lys-386' (By similarity). 

Sequence Annotation

 REGION 13 18 Interaction with SUMO1 (By similarity).
 ACT_SITE 93 93 Glycyl thioester intermediate (By
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 65 65 N6-acetyllysine (By similarity).
 MOD_RES 71 71 Phosphoserine; by CDK1 (By similarity).
 CROSSLNK 18 18 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ATP-binding; Cell cycle; Cell division; Chromosome partition; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 158 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0030425; C:dendrite; IDA:RGD.
 GO:0001650; C:fibrillar center; IDA:RGD.
 GO:0016604; C:nuclear body; IDA:RGD.
 GO:0016605; C:PML body; IEA:Compara.
 GO:0045202; C:synapse; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0019789; F:SUMO ligase activity; IDA:RGD.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:RGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0033145; P:positive regulation of intracellular steroid hormone receptor signaling pathway; IDA:RGD.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:RGD.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:RGD.
 GO:0016925; P:protein sumoylation; IDA:RGD.
 GO:0010469; P:regulation of receptor activity; IDA:RGD. 

Interpro

 IPR027230; Ubc9.
 IPR000608; UBQ-conjugat_E2.
 IPR023313; UBQ-conjugating_AS.
 IPR016135; UBQ-conjugating_enzyme/RWD. 

Pfam

 PF00179; UQ_con 

SMART

  

PROSITE

 PS00183; UBIQUITIN_CONJUGAT_1
 PS50127; UBIQUITIN_CONJUGAT_2 

PRINTS