UniProt Accession

 DHB12_HUMAN; Q53GQ0; A8K9B0; D3DR23; Q96JU2; Q9Y6G8 

Genbank Protein ID

 AF078850; AK027882; AK074952; AK075216; AK222881; AK292625; CH471064; CH471064; BC012043 

Genbank Nucleotide ID

 AAD44482.1; BAG52039.1; BAG52086.1; BAD96601.1; BAF85314.1; EAW68082.1; EAW68088.1; AAH12043.1 

Protein Name

 Estradiol 17-beta-dehydrogenase 12 

Protein Synonyms/Alias

 17-beta-hydroxysteroid dehydrogenase 12; 17-beta-HSD 12; 3-ketoacyl-CoA reductase; KAR 

Gene Name

 HSD17B12 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
84	VVLISRSKDKLDQVS	ubiquitination	[1, 2, 3]
86	LISRSKDKLDQVSSE	ubiquitination	[4, 5]
95	DQVSSEIKEKFKVET	ubiquitination	[1, 2]
117	ASEDIYDKIKTGLAG	acetylation	[6]
117	ASEDIYDKIKTGLAG	ubiquitination	[1]
181	PGMVERSKGAILNIS	ubiquitination	[1]
223	LHEEYRSKGVFVQSV	ubiquitination	[1, 4, 5]
238	LPYFVATKLAKIRKP	ubiquitination	[1, 2, 4, 5]
244	TKLAKIRKPTLDKPS	ubiquitination	[4, 5]
257	PSPETFVKSAIKTVG	ubiquitination	[4, 5]
261	TFVKSAIKTVGLQSR	ubiquitination	[1, 3, 4, 5, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. 

Sequence Annotation

 NP_BIND 50 79 NADP (By similarity).
 MOTIF 308 312 Di-lysine motif.
 ACT_SITE 202 202 Proton acceptor (By similarity).
 BINDING 189 189 Substrate (By similarity).  

Keyword

 Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Polymorphism; Reference proteome; Steroid biosynthesis; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 312 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0031012; C:extracellular matrix; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0004303; F:estradiol 17-beta-dehydrogenase activity; IEA:EC.
 GO:0008201; F:heparin binding; IEA:Compara.
 GO:0006703; P:estrogen biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0030198; P:extracellular matrix organization; IEA:Compara.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0035338; P:long-chain fatty-acyl-CoA biosynthetic process; TAS:Reactome.
 GO:0010811; P:positive regulation of cell-substrate adhesion; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0019432; P:triglyceride biosynthetic process; TAS:Reactome. 

Interpro

 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 

Pfam

 PF00106; adh_short 

SMART

  

PROSITE

 PS00061; ADH_SHORT 

PRINTS

 PR00081; GDHRDH.
 PR00080; SDRFAMILY.