CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleolar GTP-binding protein 2 
Protein Synonyms/Alias
  
Gene Name
 NOG2 
Gene Synonyms/Alias
 YNR053C; N3484 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
46LNMYTSGKEIRNKKGacetylation[1]
144YADAFGPKAQRKRPRubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation. 
Sequence Annotation
 DOMAIN 327 379 G.
 NP_BIND 322 329 GTP (Potential).
 NP_BIND 366 370 GTP (Potential).
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 155 155 Phosphoserine.  
Keyword
 Complete proteome; GTP-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 486 AA 
Protein Sequence
MGTGKKEKSR RIREGDTKDG NLRVKGENFY RDSKRVKFLN MYTSGKEIRN KKGNLIRAAS 60
FQDSTIPDAR VQPDRRWFGN TRVISQDALQ HFRSALGETQ KDTYQVLLRR NKLPMSLLEE 120
KDADESPKAR ILDTESYADA FGPKAQRKRP RLAASNLEDL VKATNEDITK YEEKQVLDAT 180
LGLMGNQEDK ENGWTSAAKE AIFSKGQSKR IWNELYKVID SSDVVIHVLD ARDPLGTRCK 240
SVEEYMKKET PHKHLIYVLN KCDLVPTWVA AAWVKHLSKE RPTLAFHASI TNSFGKGSLI 300
QLLRQFSQLH TDRKQISVGF IGYPNTGKSS IINTLRKKKV CQVAPIPGET KVWQYITLMK 360
RIFLIDCPGI VPPSSKDSEE DILFRGVVRV EHVTHPEQYI PGVLKRCQVK HLERTYEISG 420
WKDATEFIEI LARKQGRLLK GGEPDESGVS KQILNDFNRG KIPWFVLPPE KEGEEKPKKK 480
EVEKTA 486 
Gene Ontology
 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0005654; C:nucleoplasm; IDA:SGD.
 GO:0030687; C:preribosome, large subunit precursor; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; TAS:SGD.
 GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD. 
Interpro
 IPR023179; GTP-bd_ortho_bundle.
 IPR006073; GTP_binding_domain.
 IPR024929; NOG2.
 IPR012971; NOG2_N_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF01926; MMR_HSR1
 PF08153; NGP1NT 
SMART
  
PROSITE
  
PRINTS
 PR00326; GTP1OBG.