CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012487
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 43 
Protein Synonyms/Alias
  
Gene Name
 WDR43 
Gene Synonyms/Alias
 KIAA0007 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
119VKGELHSKLISGGHDubiquitination[1]
165CKVKCKWKGDNSSVSubiquitination[1]
181LCISPDGKMLLSAGRubiquitination[1, 2]
191LSAGRTIKLWVLETKubiquitination[3, 4]
198KLWVLETKEVYRHFTubiquitination[1]
384ISNCWAPKVETAITKubiquitination[1, 5]
391KVETAITKVRTPVMNubiquitination[1, 2, 5, 6, 7, 8]
452MDIDTHKKGKEDLQTubiquitination[1]
454IDTHKKGKEDLQTNSubiquitination[7, 8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
 REPEAT 11 51 WD 1.
 REPEAT 57 119 WD 2.
 REPEAT 124 163 WD 3.
 REPEAT 166 205 WD 4.
 REPEAT 207 259 WD 5.
 REPEAT 267 309 WD 6.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 394 394 Phosphothreonine.
 MOD_RES 399 399 Phosphoserine.
 MOD_RES 431 431 Phosphoserine.
 MOD_RES 437 437 Phosphoserine.
 MOD_RES 590 590 Phosphoserine.
 MOD_RES 656 656 Phosphothreonine.
 MOD_RES 658 658 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 677 AA 
Protein Sequence
MAAGGGGSCD PLAPAGVPCA FSPHSQAYFA LASTDGHLRV WETANNRLHQ EYVPSAHLSG 60
TCTCLAWAPA RLQAKESPQR KKRKSEAVGM SNQTDLLALG TAVGSILLYS TVKGELHSKL 120
ISGGHDNRVN CIQWHQDSGC LYSCSDDKHI VEWNVQTCKV KCKWKGDNSS VSSLCISPDG 180
KMLLSAGRTI KLWVLETKEV YRHFTGHATP VSSLMFTTIR PPNESQPFDG ITGLYFLSGA 240
VHDRLLNVWQ VRSENKEKSA VMSFTVTDEP VYIDLTLSEN KEEPVKLAVV CRDGQVHLFE 300
HILNGYCKKP LTSNCTIQIA TPGKGKKSTP KPIPILAAGF CSDKMSLLLV YGSWFQPTIE 360
RVALNSREPH MCLVRDISNC WAPKVETAIT KVRTPVMNSE AKVLVPGIPG HHAAIKPAPP 420
QTEQVESKRK SGGNEVSIEE RLGAMDIDTH KKGKEDLQTN SFPVLLTQGL ESNDFEMLNK 480
VLQTRNVNLI KKTVLRMPLH TIIPLLQELT KRLQGHPNSA VLMVQWLKCV LTVHASYLST 540
LPDLVPQLGT LYQLMESRVK TFQKLSHLHG KLILLITQVT ASEKTKGATS PGQKAKLVYE 600
EESSEEESDD EIADKDSEDN WDEDEEESES EKDEDVEEED EDAEGKDEEN GEDRDTASEK 660
ELNGDSDLDP ENESEEE 677 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell. 
Interpro
 IPR007148; SSU_processome_Utp12.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04003; Utp12
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS