UniProt Accession

 CDC48_YEAST; P25694; D6VRM4 

Genbank Protein ID

 X56956; Z74174; BK006938 

Genbank Nucleotide ID

 CAA40276.1; CAA98694.1; DAA11734.1 

Protein Name

 Cell division control protein 48 

Protein Synonyms/Alias

  

Gene Name

 CDC48 

Gene Synonyms/Alias

 YDL126C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
21	VDPREEDKTATAILR	ubiquitination	[1]
119	IHPCPDIKYATRISV	acetylation	[2]
119	IHPCPDIKYATRISV	ubiquitination	[1]
158	EAYRPVRKGDHFVVR	ubiquitination	[1]
221	DDIGGCRKQMAQIRE	ubiquitination	[1]
241	LRHPQLFKAIGIKPP	ubiquitination	[3]
246	LFKAIGIKPPRGVLM	ubiquitination	[1]
261	YGPPGTGKTLMARAV	ubiquitination	[1]
298	ESESNLRKAFEEAEK	ubiquitination	[1]
305	KAFEEAEKNAPAIIF	ubiquitination	[3]
322	EIDSIAPKRDKTNGE	ubiquitination	[3]
346	LTLMDGMKARSNVVV	ubiquitination	[3]
522	KFGLSPSKGVLFYGP	ubiquitination	[1, 3]
534	YGPPGTGKTLLAKAV	ubiquitination	[1]
539	TGKTLLAKAVATEVS	ubiquitination	[1, 3]
594	DELDSIAKARGGSLG	ubiquitination	[1, 3, 4, 5, 6]
624	EMDGMNAKKNVFVIG	ubiquitination	[1]
673	ILNAQLRKTPLEPGL	ubiquitination	[1, 4, 5, 6]
740	EMTDEGAKAEQEPEV	ubiquitination	[1]
755	DPVPYITKEHFAEAM	acetylation	[2]
763	EHFAEAMKTAKRSVS	acetylation	[2]
763	EHFAEAMKTAKRSVS	ubiquitination	[1]
785	EAYSQQMKASRGQFS	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [5] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [6] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269] 

Functional Description

 Involved in spindle disassembly, degradation of ubiquitinated proteins and protein export from the endoplasmic reticulum to the cytoplasm. Acts as a chaperone that collects ubiquitinated substrates. Has a role in the endoplasmic reticulum- associated degradation (ERAD) pathway. Required for the proteasome-dependent processing/activation of MGA2 and SPT23 transcription factors leading to the subsequent expression of OLE1. Has an additional role in the turnover of OLE1 where it targets ubiquitinated OLE1 and other proteins to the ERAD. 

Sequence Annotation

 NP_BIND 255 262 ATP (Potential).
 NP_BIND 528 535 ATP (Potential).
 MOD_RES 683 683 Phosphothreonine.
 CROSSLNK 594 594 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 673 673 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 ATP-binding; Cell cycle; Complete proteome; Endoplasmic reticulum; Isopeptide bond; Microsome; Nucleotide-binding; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 835 AA 

Protein Sequence

Gene Ontology

 GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:SGD.
 GO:0036266; C:Cdc48p-Npl4p-Vms1p AAA ATPase complex; IDA:SGD.
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0000837; C:Doa10p ubiquitin ligase complex; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0071629; P:cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD.
 GO:0016320; P:endoplasmic reticulum membrane fusion; IMP:SGD.
 GO:0071712; P:ER-associated misfolded protein catabolic process; IMP:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0072671; P:mitochondria-associated protein catabolic process; IMP:SGD.
 GO:0051228; P:mitotic spindle disassembly; IMP:SGD.
 GO:0071630; P:nucleus-associated proteasomal ubiquitin-dependent protein catabolic process; IMP:SGD.
 GO:0034727; P:piecemeal microautophagy of nucleus; IMP:SGD.
 GO:1900182; P:positive regulation of protein localization to nucleus; IMP:SGD.
 GO:0030970; P:retrograde protein transport, ER to cytosol; IMP:SGD.
 GO:0034517; P:ribophagy; IMP:SGD.
 GO:0031134; P:sister chromatid biorientation; IMP:SGD. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR009010; Asp_de-COase-like_dom.
 IPR005938; ATPase_AAA_CDC48.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR004201; Cdc48_dom2.
 IPR003338; CDC4_N-term_subdom.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00004; AAA
 PF02933; CDC48_2
 PF02359; CDC48_N 

SMART

 SM00382; AAA
 SM01072; CDC48_2
 SM01073; CDC48_N 

PROSITE

 PS00674; AAA 

PRINTS