UniProt Accession

 SPC25_HUMAN; Q9HBM1; A8K4X8; D3DPC0 

Genbank Protein ID

 AY456388; AF225416; CR457197; AK291093; AC069137; CH471058; CH471058; BC022255 

Genbank Nucleotide ID

 AAR88652.1; AAG09718.1; CAG33478.1; BAF83782.1; AAY24098.1; EAX11289.1; EAX11290.1; AAH22255.1 

Protein Name

 Kinetochore protein Spc25 

Protein Synonyms/Alias

 hSpc25 

Gene Name

 SPC25 

Gene Synonyms/Alias

 SPBC25; AD024 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
19	SINEFWNKFKSTDTS	ubiquitination	[1, 2, 3, 4]
21	NEFWNKFKSTDTSCQ	ubiquitination	[3]
37	AGLRDTYKDSIKAFA	ubiquitination	[3]
46	SIKAFAEKLSVKLKE	ubiquitination	[3]
50	FAEKLSVKLKEEERM	ubiquitination	[3]
78	QNKLIQEKKDNLLKL	ubiquitination	[3]
79	NKLIQEKKDNLLKLI	ubiquitination	[3]
90	LKLIAEVKGKKQELE	ubiquitination	[3]
92	LIAEVKGKKQELEVL	ubiquitination	[3]
107	TANIQDLKEEYSRKK	ubiquitination	[3]
133	ERLKRLQKSADLYKD	ubiquitination	[3]
139	QKSADLYKDRLGLEI	ubiquitination	[3]
203	EFQENVRKTNNFSAF	ubiquitination	[3, 5]
216	AFLANVRKAFTATVY	ubiquitination	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. 

Sequence Annotation

 REGION 1 144 Interaction with the N-terminus of
 REGION 1 56 Interaction with the NDC80-NUF2
 REGION 145 224 Interaction with the C-terminus of
 MOD_RES 12 12 Phosphoserine.  

Keyword

 3D-structure; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 224 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031262; C:Ndc80 complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007052; P:mitotic spindle organization; IMP:UniProtKB. 

Interpro

 IPR013255; Spc25. 

Pfam

 PF08234; Spindle_Spc25 

SMART

  

PROSITE

  

PRINTS