UniProt Accession

 RASF1_HUMAN; Q9NS23; B7ZLL1; O14571; O60539; O60710; Q0VGC6; Q5TZT2; Q9HB04; Q9HB18; Q9NS22; Q9UND4; Q9UND5 

Genbank Protein ID

 AF061836; AF132675; AF132676; AF132677; AF040703; AF102770; AF102771; AF102772; BT020047; BT020048; AF286217; AF291719; AC002455; AC002481; CH471055; CH471055; CH471055; BC110412; BC143879 

Genbank Nucleotide ID

 AAC16001.1; AAD44174.1; AAD44175.1; AAD44176.1; AAC70910.2; AAF35127.2; AAF35128.2; AAF35129.2; AAV38850.1; AAV38851.1; AAG10038.1; AAG10064.1; AAB67312.1; EAW65098.1; EAW65101.1; EAW65102.1; AAI10413.1; AAI43880.1 

Protein Name

 Ras association domain-containing protein 1 

Protein Synonyms/Alias

  

Gene Name

 RASSF1 

Gene Synonyms/Alias

 RDA32 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
236	VIEALLRKFLVVDDP	ubiquitination	[1]

Reference

 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. 

Sequence Annotation

 DOMAIN 198 292 Ras-associating.
 DOMAIN 294 341 SARAH.
 ZN_FING 51 105 Phorbol-ester/DAG-type.
 REGION 2 119 Mediates interaction with E4F1.
 REGION 315 318 MOAP1-binding.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative promoter usage; Alternative splicing; Cell cycle; Complete proteome; Cytoplasm; Cytoskeleton; Metal-binding; Microtubule; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Tumor suppressor; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 344 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0015630; C:microtubule cytoskeleton; IDA:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; TAS:UniProtKB.
 GO:0007050; P:cell cycle arrest; IMP:UniProtKB.
 GO:0071157; P:negative regulation of cell cycle arrest; IDA:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IDA:UniProtKB.
 GO:0007265; P:Ras protein signal transduction; IDA:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IMP:UniProtKB. 

Interpro

 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR000159; Ras-assoc.
 IPR011524; SARAH_dom. 

Pfam

 PF00130; C1_1
 PF00788; RA 

SMART

 SM00109; C1
 SM00314; RA 

PROSITE

 PS50200; RA
 PS50951; SARAH
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 

PRINTS