CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003798
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial-processing peptidase subunit beta 
Protein Synonyms/Alias
 Beta-MPP; PEP 
Gene Name
 MAS1 
Gene Synonyms/Alias
 MIF1; YLR163C; L9632.10 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
185RTILGPIKNIKSITRacetylation[1]
196SITRTDLKDYITKNYacetylation[1]
220AGAVDHEKLVQYAQKacetylation[1]
227KLVQYAQKYFGHVPKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Cleaves presequences (transit peptides) from mitochondrial protein precursors. 
Sequence Annotation
 ACT_SITE 73 73 Proton acceptor.
 METAL 70 70 Zinc.
 METAL 74 74 Zinc.
 METAL 150 150 Zinc.
 MOD_RES 243 243 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Mitochondrion; Phosphoprotein; Protease; Reference proteome; Transit peptide; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 462 AA 
Protein Sequence
MFSRTASKFR NTRRLLSTIS SQIPGTRTSK LPNGLTIATE YIPNTSSATV GIFVDAGSRA 60
ENVKNNGTAH FLEHLAFKGT QNRSQQGIEL EIENIGSHLN AYTSRENTVY YAKSLQEDIP 120
KAVDILSDIL TKSVLDNSAI ERERDVIIRE SEEVDKMYDE VVFDHLHEIT YKDQPLGRTI 180
LGPIKNIKSI TRTDLKDYIT KNYKGDRMVL AGAGAVDHEK LVQYAQKYFG HVPKSESPVP 240
LGSPRGPLPV FCRGERFIKE NTLPTTHIAI ALEGVSWSAP DYFVALATQA IVGNWDRAIG 300
TGTNSPSPLA VAASQNGSLA NSYMSFSTSY ADSGLWGMYI VTDSNEHNVQ LIVNEILKEW 360
KRIKSGKISD AEVNRAKAQL KAALLLSLDG STAIVEDIGR QVVTTGKRLS PEEVFEQVDK 420
ITKDDIIMWA NYRLQNKPVS MVALGNTSTV PNVSYIEEKL NQ 462 
Gene Ontology
 GO:0017087; C:mitochondrial processing peptidase complex; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; TAS:Reactome.
 GO:0006627; P:protein processing involved in protein targeting to mitochondrion; IDA:SGD.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR011249; Metalloenz_LuxS/M16.
 IPR011237; Pept_M16_dom.
 IPR011765; Pept_M16_N.
 IPR001431; Pept_M16_Zn_BS.
 IPR007863; Peptidase_M16_C. 
Pfam
 PF00675; Peptidase_M16
 PF05193; Peptidase_M16_C 
SMART
  
PROSITE
 PS00143; INSULINASE 
PRINTS