CPLM-023601

Genbank Nucleotide ID

Proteasome maturation protein

Protein Synonyms/Alias

Proteassemblin; Protein UMP1 homolog; hUMP1; Voltage-gated K channel beta subunit 4.1

C13orf12; UMP1; HSPC014; HSPC036; PNAS-110

Homo sapiens (Human)

Position	Peptide	Type	References
11	RGLGSELKDSIPVTE	ubiquitination	[1, 2, 3]
33	ESHDLLRKGFSCVKN	ubiquitination	[1, 2]
39	RKGFSCVKNELLPSH	ubiquitination	[2, 3, 4, 5]
53	HPLELSEKNFQLNQD	ubiquitination	[1, 2, 3, 4, 6, 7, 8]
61	NFQLNQDKMNFSTLR	ubiquitination	[1, 2, 3, 5, 6, 7, 8]
78	QGLFAPLKLQMEFKA	ubiquitination	[4, 7]
84	LKLQMEFKAVQQVQR	ubiquitination	[1, 2, 7]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.

MOTIF 68 72 High-affinity association with the

Chaperone; Complete proteome; Cytoplasm; Direct protein sequencing; Endoplasmic reticulum; Ichthyosis; Membrane; Microsome; Nucleus; Palmoplantar keratoderma; Proteasome; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
GO:0005634; C:nucleus; IDA:HPA.
GO:0031090; C:organelle membrane; IEA:UniProtKB-SubCell.
GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
GO:0043248; P:proteasome assembly; IMP:UniProtKB.