CPLM-021979

Genbank Nucleotide ID

Mediator of RNA polymerase II transcription subunit 4

Protein Synonyms/Alias

Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36

ARC36; DRIP36; VDRIP; HSPC126

Homo sapiens (Human)

Position	Peptide	Type	References
55	LAISRNQKLLQAGEE	ubiquitination	[1, 2, 3, 4]
81	GEFQELMKLALNQGK	ubiquitination	[1, 2, 3, 4]
88	KLALNQGKIHHEMQV	ubiquitination	[2]
98	HEMQVLEKEVEKRDS	ubiquitination	[2]
112	SDIQQLQKQLKEAEQ	ubiquitination	[1, 2, 3, 4, 5]
131	AVYQAKEKLKSIEKA	ubiquitination	[2]
140	KSIEKARKGAISSEE	ubiquitination	[2]
150	ISSEEIIKYAHRISA	ubiquitination	[1, 2, 4, 5, 6]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

MOD_RES 32 32 Phosphoserine.

Activator; Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016592; C:mediator complex; IDA:UniProtKB.
GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; NAS:UniProtKB.
GO:0004872; F:receptor activity; IDA:UniProtKB.
GO:0001104; F:RNA polymerase II transcription cofactor activity; IDA:UniProtKB.
GO:0046966; F:thyroid hormone receptor binding; IDA:UniProtKB.
GO:0042809; F:vitamin D receptor binding; NAS:UniProtKB.
GO:0030521; P:androgen receptor signaling pathway; IDA:UniProtKB.
GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:UniProtKB.

IPR019258; Mediator_Med4.