CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012481
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 6 
Protein Synonyms/Alias
 Alpha-Pix; COOL-2; PAK-interacting exchange factor alpha; Rac/Cdc42 guanine nucleotide exchange factor 6 
Gene Name
 ARHGEF6 
Gene Synonyms/Alias
 COOL2; KIAA0006; PIXA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22GVLESPKKTICDPEEubiquitination[1]
32CDPEEFLKSSLKNGVubiquitination[1]
43KNGVVLCKLINRLMPubiquitination[1]
108STLLAVNKATEDQLSubiquitination[1]
151SGLQRQSKTVEMTENubiquitination[1]
166GSHQLIVKARFNFKQubiquitination[1]
227SERPLSPKAVKGFETubiquitination[1]
230PLSPKAVKGFETAPLubiquitination[1]
382KPFMRLEKYVTLLQEubiquitination[1]
495SGFIYQGKIPIAGTVubiquitination[1]
558ASCSSLSKTSSSSCSubiquitination[1, 2]
617ERMSYILKESSKSPKubiquitination[1]
638HKRKTERKPSEEEYVubiquitination[1]
648EEEYVIRKSTAALEEubiquitination[1]
661EEDAQILKVIEAYCTubiquitination[1]
682GHGSSTRKDSIPQVLubiquitination[1]
695VLLPEEEKLIIEETRubiquitination[1]
712GQTIMEEKSLVDTVYubiquitination[1]
722VDTVYALKDEVRELKubiquitination[1, 2]
736KQENKRMKQCLEEELubiquitination[1]
751KSRRDLEKLVRRLLKacetylation[3]
758KLVRRLLKQTDECIRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Acts as a RAC1 guanine nucleotide exchange factor (GEF). 
Sequence Annotation
 DOMAIN 1 110 CH.
 DOMAIN 160 219 SH3.
 DOMAIN 241 421 DH.
 DOMAIN 443 548 PH.
 MOD_RES 122 122 Phosphoserine (By similarity).
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 144 144 Phosphoserine.
 MOD_RES 150 150 Phosphoserine.
 MOD_RES 488 488 Phosphoserine (By similarity).
 MOD_RES 640 640 Phosphoserine.
 MOD_RES 650 650 Phosphothreonine (By similarity).
 MOD_RES 684 684 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Cell projection; Complete proteome; Guanine-nucleotide releasing factor; Mental retardation; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 776 AA 
Protein Sequence
MNPEEQIVTW LISLGVLESP KKTICDPEEF LKSSLKNGVV LCKLINRLMP GSVEKFCLDP 60
QTEADCINNI NDFLKGCATL QVEIFDPDDL YSGVNFSKVL STLLAVNKAT EDQLSERPCG 120
RSSSLSAANT SQTNPQGAVS STVSGLQRQS KTVEMTENGS HQLIVKARFN FKQTNEDELS 180
VCKGDIIYVT RVEEGGWWEG TLNGRTGWFP SNYVREIKSS ERPLSPKAVK GFETAPLTKN 240
YYTVVLQNIL DTEKEYAKEL QSLLVTYLRP LQSNNNLSTV EVTSLLGNFE EVCTFQQTLC 300
QALEECSKFP ENQHKVGGCL LSLMPHFKSM YLAYCANHPS AVNVLTQHSD ELEQFMENQG 360
ASSPGILILT TNLSKPFMRL EKYVTLLQEL ERHMEDTHPD HQDILKAIVA FKTLMGQCQD 420
LRKRKQLELQ ILSEPIQAWE GEDIKNLGNV IFMSQVMVQY GACEEKEERY LMLFSNVLIM 480
LSASPRMSGF IYQGKIPIAG TVVTRLDEIE GNDCTFEITG NTVERIVVHC NNNQDFQEWL 540
EQLNRLIRGP ASCSSLSKTS SSSCSAHSSF SSTGQPRGPL EPPQIIKPWS LSCLRPAPPL 600
RPSAALGYKE RMSYILKESS KSPKTMKKFL HKRKTERKPS EEEYVIRKST AALEEDAQIL 660
KVIEAYCTSA NFQQGHGSST RKDSIPQVLL PEEEKLIIEE TRSNGQTIME EKSLVDTVYA 720
LKDEVRELKQ ENKRMKQCLE EELKSRRDLE KLVRRLLKQT DECIRGESSS KTSILP 776 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0005096; F:GTPase activator activity; NAS:UniProtKB.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; NAS:UniProtKB.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0034329; P:cell junction assembly; TAS:Reactome.
 GO:0007254; P:JNK cascade; NAS:UniProtKB.
 GO:0030032; P:lamellipodium assembly; ISS:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR001715; CH-domain.
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR003096; SM22_calponin. 
Pfam
 PF00307; CH
 PF00169; PH
 PF00621; RhoGEF
 PF07653; SH3_2 
SMART
 SM00033; CH
 SM00233; PH
 SM00325; RhoGEF
 SM00326; SH3 
PROSITE
 PS50021; CH
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.
 PR00888; SM22CALPONIN.