CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-045079
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Phenylalanine--tRNA ligase alpha subunit 
Protein Synonyms/Alias
  
Gene Name
 FARSA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
79RSELRKRKLLAEVTLubiquitination[1]
93LKTYWVSKGSAFSTSubiquitination[1, 2, 3, 4]
103AFSTSISKQETELSPubiquitination[1, 2, 3, 4, 5, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 379 AA 
Protein Sequence
XAELLLRRLE ASDGGLDSAE LAAELGMEHQ AVVGAVKSLQ ALGEVDSMED EVQRRLQLVR 60
GGQAEKLGEK ERSELRKRKL LAEVTLKTYW VSKGSAFSTS ISKQETELSP EMISSGSWRD 120
RPFKPYNFLA HGVLPDSGHL HPLLKVRSQF RQIFLEMGFT EMPTDNFIES SFWNFDALFQ 180
PQQHPARDQH DTFFLRDPAE ALQLPMDYVQ RVKRTHSQGG YGSQGYKYNW KLDEARKNLL 240
RTHTTSASAR ALYRLAQKKP FTPVKYFSID RVFRNETLDA THLAEFHQIE GVVADHGLTL 300
GHLMGVLREF FTKLGITQLR FKPAYNPYTE PSMEVFSYHQ GLKKWVEVGN SGVFRPEMLL 360
PMGLPENVSV IAWGLSLER 379 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004826; F:phenylalanine-tRNA ligase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:InterPro.
 GO:0006432; P:phenylalanyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR006195; aa-tRNA-synth_II.
 IPR004529; Phe-tRNA-synth_IIc_asu.
 IPR002319; Phenylalanyl-tRNA_Synthase. 
Pfam
 PF01409; tRNA-synt_2d 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS