UniProt Accession

 B4DDD8_HUMAN; B4DDD8 

Genbank Protein ID

 AC116353; AK293154 

Genbank Nucleotide ID

 BAG56699.1 

Protein Name

 Histidine--tRNA ligase, cytoplasmic 

Protein Synonyms/Alias

 cDNA FLJ53703, highly similar to Histidyl-tRNA synthetase (EC 6.1.1.21) 

Gene Name

 HARS 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
12	AALEELVKLQGERVR	ubiquitination	[1]
42	VAKLLKLKAQLGPDE	ubiquitination	[1, 2]
51	QLGPDESKQKFVLKT	ubiquitination	[2]
53	GPDESKQKFVLKTPK	ubiquitination	[1]
57	SKQKFVLKTPKGTRD	ubiquitination	[1, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

  

Sequence Annotation

  

Keyword

 Aminoacyl-tRNA synthetase; Complete proteome; Ligase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 435 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0004812; F:aminoacyl-tRNA ligase activity; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro. 

Interpro

 IPR004154; Anticodon-bd.
 IPR004516; HisRS/HisZ.
 IPR009068; S15_NS1_RNA-bd.
 IPR000738; WHEP-TRS. 

Pfam

 PF03129; HGTP_anticodon
 PF00458; WHEP-TRS 

SMART

 SM00991; WHEP-TRS 

PROSITE

 PS00762; WHEP_TRS_1
 PS51185; WHEP_TRS_2 

PRINTS