UniProt Accession

 CBIK_SALTY; Q05592 

Genbank Protein ID

 L12006; AE006468 

Genbank Nucleotide ID

 AAA27262.1; AAL20929.1 

Protein Name

 Sirohydrochlorin cobaltochelatase 

Protein Synonyms/Alias

  

Gene Name

 cbiK 

Gene Synonyms/Alias

 STM2025 

Created Date

 July 27, 2013 

Organism

 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 

NCBI Taxa ID

 99287 

Lysine Modification

Position	Peptide	Type	References
2	******MKKALLVVS	acetylation	[1]
3	*****MKKALLVVSF	acetylation	[1]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. 

Sequence Annotation

 REGION 85 92 Substrate binding.
 REGION 202 203 Substrate binding.
 ACT_SITE 145 145 Proton acceptor (Potential).
 METAL 145 145 Cobalt.
 METAL 175 175 Cobalt (Probable).
 METAL 207 207 Cobalt (Probable).
 BINDING 10 10 Substrate.
 BINDING 207 207 Substrate.  

Keyword

 3D-structure; Cobalamin biosynthesis; Cobalt; Complete proteome; Direct protein sequencing; Lyase; Metal-binding; Porphyrin biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 264 AA 

Protein Sequence

Gene Ontology

 GO:0050897; F:cobalt ion binding; IDA:UniProtKB.
 GO:0016852; F:sirohydrochlorin cobaltochelatase activity; IEA:EC.
 GO:0046906; F:tetrapyrrole binding; IDA:UniProtKB.
 GO:0019251; P:anaerobic cobalamin biosynthetic process; IEA:InterPro.
 GO:0006779; P:porphyrin-containing compound biosynthetic process; IEA:UniProtKB-KW. 

Interpro

 IPR010388; Anaerobic_Co-chelatase. 

Pfam

 PF06180; CbiK 

SMART

  

PROSITE

  

PRINTS