UniProt Accession

 PIL1_YEAST; P53252; D6VUL8 

Genbank Protein ID

 Z72871; BK006941 

Genbank Nucleotide ID

 CAA97088.1; DAA08179.1 

Protein Name

 Sphingolipid long chain base-responsive protein PIL1 

Protein Synonyms/Alias

  

Gene Name

 PIL1 

Gene Synonyms/Alias

 YGR086C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
29	PPPPSTTKGRFFGKG	ubiquitination	[1, 2]
35	TKGRFFGKGGLAYSF	acetylation	[3]
57	FGPELSRKLSQLVKI	ubiquitination	[2]
63	RKLSQLVKIEKNVLR	acetylation	[3]
66	SQLVKIEKNVLRSME	acetylation	[3]
66	SQLVKIEKNVLRSME	ubiquitination	[2]
84	NERRDAAKQLSIWGL	ubiquitination	[2]
130	DQYRLTLKSIRDIEG	ubiquitination	[1, 2]
152	RKDKITDKIAYLKYK	acetylation	[3]
152	RKDKITDKIAYLKYK	ubiquitination	[2]
165	YKDPQSPKIEVLEQE	acetylation	[3]
165	YKDPQSPKIEVLEQE	ubiquitination	[1]
212	SIIEHSEKIALIAGY	ubiquitination	[2]
221	ALIAGYGKALLELLD	ubiquitination	[2]

Reference

 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Negative regulator of cell wall integrity (CWI) in unstressed cells, probably by inhibiting protein kinase PKH1/PHK2 activity and regulating their downstream CWI pathways PKC1-MAP kinase pathway and protein kinase YPK1 pathway. Activity may be regulated by the transient increase of sphingolipid long chain bases (LCBs) during heat stress. 

Sequence Annotation

 MOD_RES 14 14 Phosphothreonine.
 MOD_RES 16 16 Phosphoserine.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 163 163 Phosphoserine.
 MOD_RES 230 230 Phosphoserine.
 MOD_RES 233 233 Phosphothreonine.
 MOD_RES 299 299 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; Lipid droplet; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 339 AA 

Protein Sequence

Gene Ontology

 GO:0032126; C:eisosome; IDA:SGD.
 GO:0005811; C:lipid particle; IEA:UniProtKB-SubCell.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0008289; F:lipid binding; IDA:SGD.
 GO:0070941; P:eisosome assembly; IMP:SGD.
 GO:0006897; P:endocytosis; IGI:SGD.
 GO:0006469; P:negative regulation of protein kinase activity; IMP:SGD.
 GO:0008104; P:protein localization; IMP:SGD.
 GO:0009408; P:response to heat; IMP:SGD. 

Interpro

  

Pfam

  

SMART

  

PROSITE

  

PRINTS