CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033486
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Apoptotic protease-activating factor 1 
Protein Synonyms/Alias
  
Gene Name
 APAF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
198DKSGLLMKLQNLCTRubiquitination[1]
224PLNIEEAKDRLRILMubiquitination[1, 2]
267ILLTTRDKSVTDSVMubiquitination[1]
277TDSVMGPKYVVPVESubiquitination[1]
288PVESSLGKEKGLEILubiquitination[2]
290ESSLGKEKGLEILSLubiquitination[1]
380EMLREDIKDYYTDLSubiquitination[1]
643DKTLQVFKAETGEKLubiquitination[1]
733KLWDLNQKECRNTMFubiquitination[1, 3]
756CRFSPDDKLLASCSAubiquitination[1]
768CSADGTLKLWDATSAubiquitination[1]
820IMVAAKNKIFLWNTDubiquitination[4]
873WETKKVCKNSAVMLKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1163 AA 
Protein Sequence
MDAKARNCLL QHREALEKDI KTSYIMDHMI SDGFLTISEE EKVRNEPTQQ QRAAMLIKMI 60
LKKDNDSYVS FYNALLHEGY KDLAALLHDG IPVVSSSSGK DSVSGITSYV RTVLCEGGVP 120
QRPVVFVTRK KLVNAIQQKL SKLKGEPGWV TIHGMAGCGK SVLAAEAVRD HSLLEGCFPG 180
GVHWVSVGKQ DKSGLLMKLQ NLCTRLDQDE SFSQRLPLNI EEAKDRLRIL MLRKHPRSLL 240
ILDDVWDSWV LKAFDSQCQI LLTTRDKSVT DSVMGPKYVV PVESSLGKEK GLEILSLFVN 300
MKKADLPEQA HSIIKECKGS PLVVSLIGAL LRDFPNRWEY YLKQLQNKQF KRIRKSSSYD 360
YEALDEAMSI SVEMLREDIK DYYTDLSILQ KDVKVPTKVL CILWDMETEE VEDILQEFVN 420
KSLLFCDRNG KSFRYYLHDL QVDFLTEKNC SQLQDLHKKI ITQFQRYHQP HTLSPDQEDC 480
MYWYNFLAYH MASAKMHKEL CALMFSLDWI KAKTELVGPA HLIHEFVEYR HILDEKDCAV 540
SENFQEFLSL NGHLLGRQPF PNIVQLGLCE PETSEVYQQA KLQAKQEVDN GMLYLEWINK 600
KNITNLSRLV VRPHTDAVYH ACFSEDGQRI ASCGADKTLQ VFKAETGEKL LEIKAHEDEV 660
LCCAFSTDDR FIATCSVDKK VKIWNSMTGE LVHTYDEHSE QVNCCHFTNS SHHLLLATGS 720
SDCFLKLWDL NQKECRNTMF GHTNSVNHCR FSPDDKLLAS CSADGTLKLW DATSANERKS 780
INVKQFFLNL EDPQEDMEVI VKCCSWSADG ARIMVAAKNK IFLWNTDSRS KVADCRGHLS 840
WVHGVMFSPD GSSFLTSSDD QTIRLWETKK VCKNSAVMLK QEVDVVFQEN EVMVLAVDHI 900
RRLQLINGRT GQIDYLTEAQ VSCCCLSPHL QYIAFGDENG AIEILELVNN RIFQSRFQHK 960
KTVWHIQFTA DEKTLISSSD DAEIQVWNWQ LDKCIFLRGH QETVKDFRLL KNSRLLSWSF 1020
DGTVKVWNII TGNKEKDFVC HQGTVLSCDI SHDATKFSST SADKTAKIWN VSNGELLHLC 1080
APLSEEGAAT HGGWVTDLCF SPDGKMLISA GGYIKWWNVV TGESSQTFYT NGTNLKKIHV 1140
SPDFKTYVTV DNLGILYILQ TLE 1163 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0043531; F:ADP binding; IEA:InterPro.
 GO:0006952; P:defense response; IEA:InterPro.
 GO:0042981; P:regulation of apoptotic process; IEA:InterPro. 
Interpro
 IPR001315; CARD.
 IPR011029; DEATH-like_dom.
 IPR000767; Disease_R.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR002182; NB-ARC.
 IPR027417; P-loop_NTPase.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00619; CARD
 PF00931; NB-ARC
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS50209; CARD
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00364; DISEASERSIST.
 PR00320; GPROTEINBRPT.