CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009402
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heterogeneous nuclear ribonucleoprotein K 
Protein Synonyms/Alias
 hnRNP K 
Gene Name
 Hnrnpk 
Gene Synonyms/Alias
 Hnrpk 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
34MEEEQAFKRSRNTDEubiquitination[1]
52LRILLQSKNAGAVIGubiquitination[1]
60NAGAVIGKGGKNIKAubiquitination[1]
66GKGGKNIKALRTDYNubiquitination[1]
139AGGIIGVKGAKIKELubiquitination[1]
155ENTQTTIKLFQECCPubiquitination[1]
174RVVLIGGKPDRVVECubiquitination[1]
195LISESPIKGRAQPYDubiquitination[1]
372TTQVTIPKDLAGSIIubiquitination[1]
381LAGSIIGKGGQRIKQubiquitination[1]
398HESGASIKIDEPLEGubiquitination[1]
432YLLQNSVKQYSGKFFubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single- stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest. 
Sequence Annotation
 DOMAIN 42 104 KH 1.
 REPEAT 54 76 1-1.
 REPEAT 59 62 3-1.
 DOMAIN 144 209 KH 2.
 REPEAT 245 250 2-1.
 REPEAT 257 260 3-2.
 REPEAT 267 270 3-3.
 REPEAT 295 298 3-4.
 REPEAT 324 329 2-2.
 DOMAIN 387 451 KH 3.
 REPEAT 399 421 1-2.
 REPEAT 404 407 3-5.
 REGION 1 276 Necessary for interaction with DDX1 (By
 REGION 54 421 2 X 22 AA approximate repeats.
 REGION 59 407 5 X 4 AA repeats of G-X-G-G.
 REGION 209 337 Interaction with ZIK1.
 REGION 236 273 RNA-binding RGG-box.
 REGION 245 329 2 X 6 AA approximate repeats.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 116 116 Phosphoserine (By similarity).
 MOD_RES 214 214 Phosphoserine (By similarity).
 MOD_RES 216 216 Phosphoserine.
 MOD_RES 284 284 Phosphoserine.
 MOD_RES 296 296 Omega-N-methylated arginine (By
 MOD_RES 299 299 Omega-N-methylated arginine (By
 MOD_RES 379 379 Phosphoserine (By similarity).
 MOD_RES 380 380 Phosphotyrosine (By similarity).
 CROSSLNK 422 422 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Activator; Alternative splicing; Cell junction; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Glycoprotein; Isopeptide bond; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Ribonucleoprotein; RNA-binding; Spliceosome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 463 AA 
Protein Sequence
METEQPEETF PNTETNGEFG KRPAEDMEEE QAFKRSRNTD EMVELRILLQ SKNAGAVIGK 60
GGKNIKALRT DYNASVSVPD SSGPERILSI SADIETIGEI LKKIIPTLEE YQHYKGSDFD 120
CELRLLIHQS LAGGIIGVKG AKIKELRENT QTTIKLFQEC CPHSTDRVVL IGGKPDRVVE 180
CIKIILDLIS ESPIKGRAQP YDPNFYDETY DYGGFTMMFD DRRGRPVGFP MRGRGGFDRM 240
PPGRGGRPMP PSRRDYDDMS PRRGPPPPPP GRGGRGGSRA RNLPLPPPPP PRGGDLMAYD 300
RRGRPGDRYD GMVGFSADET WDSAIDTWSP SEWQMAYEPQ GGSGYDYSYA GGRGSYGDLG 360
GPIITTQVTI PKDLAGSIIG KGGQRIKQIR HESGASIKID EPLEGSEDRI ITITGTQDQI 420
QNAQYLLQNS VKQYSGKFF 439 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000790; C:nuclear chromatin; IEA:Compara.
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0002102; C:podosome; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IDA:MGI.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0045716; P:positive regulation of low-density lipoprotein particle receptor biosynthetic process; IEA:Compara.
 GO:0048260; P:positive regulation of receptor-mediated endocytosis; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; IMP:MGI.
 GO:0072369; P:regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0010988; P:regulation of low-density lipoprotein particle clearance; IEA:Compara.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1.
 IPR012987; ROK_N. 
Pfam
 PF00013; KH_1
 PF08067; ROKNT 
SMART
 SM00322; KH 
PROSITE
 PS50084; KH_TYPE_1 
PRINTS