CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013920
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoribosylformylglycinamidine synthase 
Protein Synonyms/Alias
 FGAM synthase; FGAMS; Formylglycinamide ribotide amidotransferase; FGARAT; Formylglycinamide ribotide synthetase 
Gene Name
 Pfas 
Gene Synonyms/Alias
 Kiaa0361 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
279NSSAIQGKKVKFLRPubiquitination[1]
436GIGSMEAKHVGKKPPubiquitination[1]
638VLGKMPQKEFFLQRKacetylation[2]
1031PRCVIEEKQGLKERTubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
  
Sequence Annotation
 DOMAIN 1063 1301 Glutamine amidotransferase type-1.
 NP_BIND 322 333 ATP (Potential).
 ACT_SITE 1157 1157 For GATase activity (By similarity).
 MOD_RES 619 619 Phosphothreonine (By similarity).
 MOD_RES 622 622 Phosphothreonine (By similarity).  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Glutamine amidotransferase; Ligase; Nucleotide-binding; Phosphoprotein; Purine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1337 AA 
Protein Sequence
MAPVLHFYVR PSGHEGAASG RVFRRLQEKL PTLQSVETEL CYNVHWAAET LPWAEEMKKL 60
MWLFGCPLVR DDVAQEPWLV PGSNDLLLEV GPRLNFSTPA STNIVSVCQA AGLRAVDRVE 120
TTRRYRLSFT DHPTAEMEAI SLAALHDRMT EQHYPDPIQS FSPQSIPAPL KGSIDILAEG 180
RPALEKANQE LGLALDSWDL DFYTKRFQEL QRNPSTVEVF DLAQSNSEHS RHWFFKGQLH 240
VDGKKLAHSL FESIMSTQAS SNPNNVLKFC DNSSAIQGKK VKFLRPEDST RPSCFQQQQG 300
LRHVVFTAET HNFPTGVAPF SGATTGTGGR IRDVQCTGRG AHVVAGTAGY CFGNLHIPDY 360
NLPWEDPSFQ YPGNFARPLE VAIEASNGAS DYGNKFGEPV LAGFARSLGL QLPDGQRREW 420
IKPIMFSGGI GSMEAKHVGK KPPEPGMEVV KVGGPVYRIG VGGGAASSVQ VQGDNTSDLD 480
FGAVQRGDPE MEQKMNRVIR ACVEAPGGNP ICSLHDQGAG GNGNVLKELS DPEGAIIYTS 540
RFQLGDPTLN ALEIWGAEYQ ESNALLLRPS DRDFLSRASA RERCPACFVG TITGDKRIVL 600
VDDRECLVGK TGQGDAPLTP PTPVDLDLDW VLGKMPQKEF FLQRKPPVLQ PLALPPELSV 660
RQALNRVLRL PAVASKRYLT NKVDRSVGGL VAQQQCVGPL QTPLADVAVV ALSHQECIGA 720
ATALGEQPVK SLLDPKAAAR LAVSEALTNL VFALVTDLRD VKCSGNWMWA AKLPGEGAAL 780
ADACEAMVAV MAALGVAVDG GKDSLSMAAR VGTETVQAPG SLVISAYAVC PDITATVTPD 840
LKHPGGKGHL LYVPLSPGQH RLGGTALAQC FSQLGEHPPD LDLPENLVRA FHITQGLLKE 900
CRLCSGHDVS DGGLVTCLLE MAFAGNCGIE VDVPAPGIHA LPVLFAEEPG LVLEVQEADV 960
AGVRQRYESA GLRCLELGHT GEAGPQAMAR ISVNKAVVVE EPVGELRALW EETSFQLDLL 1020
QAEPRCVIEE KQGLKERTGP SYYLPPTFPV ASVPCKPGGP VPRVAILREE GSNGDREMAD 1080
AFHLAGFEVW DVTMQDLCSG AIRLDTFRGV AFVGGFSYAD VLGSAKGWAA AVTFNPQARE 1140
ELGRFRRRPD TFSLGVCNGC QLLALLGWVG SDPSEEQAEP GQDSQPTQPG LLLRHNLSGR 1200
FESRWATVRV EPGPALMLRG MEGSVLPVWS AHGEGYMAFS SPELQAKIEA KGLVPLHWAD 1260
DDGNPTEQYP LNPNGSPGGI AGICSQDGRH LALMPHPERA VRLWQWAWRP SPFDVLPTSP 1320
WLQLFINARN WTQEDSC 1337 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004642; F:phosphoribosylformylglycinamidine synthase activity; IEA:EC.
 GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
 GO:0042493; P:response to drug; IEA:Compara. 
Interpro
 IPR010918; AIR_synth_C_dom.
 IPR000728; AIR_synth_N_dom.
 IPR017926; GATASE.
 IPR010073; PRibForGlyAmidine_synth.
 IPR016188; PurM_N-like. 
Pfam
 PF00586; AIRS
 PF02769; AIRS_C 
SMART
  
PROSITE
 PS51273; GATASE_TYPE_1 
PRINTS