CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019769
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA endonuclease RBBP8 
Protein Synonyms/Alias
 CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2 
Gene Name
 RBBP8 
Gene Synonyms/Alias
 CTIP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
432GKDSNTDKHLEPLKSacetylation[1]
526VTLYEALKTIPKGFSacetylation[1]
604ENVLDDIKSAGSHEPacetylation[1]
Reference
 [1] Human SIRT6 promotes DNA end resection through CtIP deacetylation.
 Kaidi A, Weinert BT, Choudhary C, Jackson SP.
 Science. 2010 Sep 10;329(5997):1348-53. [PMID: 20829486] 
Functional Description
 Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations. 
Sequence Annotation
 REGION 22 45 Essential for binding to the MRN complex
 REGION 509 557 Damage-recruitment motif.
 MOTIF 490 494 PXDLS motif.
 MOD_RES 326 326 Phosphoserine.
 MOD_RES 327 327 Phosphoserine.
 MOD_RES 349 349 Phosphoserine.
 MOD_RES 432 432 N6-acetyllysine.
 MOD_RES 526 526 N6-acetyllysine.
 MOD_RES 604 604 N6-acetyllysine.
 MOD_RES 664 664 Phosphoserine; by ATM.
 MOD_RES 679 679 Phosphoserine.
 MOD_RES 723 723 Phosphoserine.
 MOD_RES 745 745 Phosphoserine; by ATM.
 MOD_RES 847 847 Phosphothreonine; by CDK1.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Cell cycle; Cell division; Coiled coil; Complete proteome; DNA damage; DNA repair; DNA-binding; Dwarfism; Endonuclease; Hydrolase; Meiosis; Mental retardation; Mitosis; Nuclease; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 897 AA 
Protein Sequence
MNISGSSCGS PNSADTSSDF KDLWTKLKEC HDREVQGLQV KVTKLKQERI LDAQRLEEFF 60
TKNQQLREQQ KVLHETIKVL EDRLRAGLCD RCAVTEEHMR KKQQEFENIR QQNLKLITEL 120
MNERNTLQEE NKKLSEQLQQ KIENDQQHQA AELECEEDVI PDSPITAFSF SGVNRLRRKE 180
NPHVRYIEQT HTKLEHSVCA NEMRKVSKSS THPQHNPNEN EILVADTYDQ SQSPMAKAHG 240
TSSYTPDKSS FNLATVVAET LGLGVQEESE TQGPMSPLGD ELYHCLEGNH KKQPFEESTR 300
NTEDSLRFSD STSKTPPQEE LPTRVSSPVF GATSSIKSGL DLNTSLSPSL LQPGKKKHLK 360
TLPFSNTCIS RLEKTRSKSE DSALFTHHSL GSEVNKIIIQ SSNKQILINK NISESLGEQN 420
RTEYGKDSNT DKHLEPLKSL GGRTSKRKKT EEESEHEVSC PQASFDKENA FPFPMDNQFS 480
MNGDCVMDKP LDLSDRFSAI QRQEKSQGSE TSKNKFRQVT LYEALKTIPK GFSSSRKASD 540
GNCTLPKDSP GEPCSQECII LQPLNKCSPD NKPSLQIKEE NAVFKIPLRP RESLETENVL 600
DDIKSAGSHE PIKIQTRSDH GGCELASVLQ LNPCRTGKIK SLQNNQDVSF ENIQWSIDPG 660
ADLSQYKMDV TVIDTKDGSQ SKLGGETVDM DCTLVSETVL LKMKKQEQKG EKSSNEERKM 720
NDSLEDMFDR TTHEEYESCL ADSFSQAADE EEELSTATKK LHTHGDKQDK VKQKAFVEPY 780
FKGDERETSL QNFPHIEVVR KKEERRKLLG HTCKECEIYY ADMPAEEREK KLASCSRHRF 840
RYIPPNTPEN FWEVGFPSTQ TCMERGYIKE DLDPCPRPKR RQPYNAIFSP KGKEQKT 897 
Gene Ontology
 GO:0017053; C:transcriptional repressor complex; IDA:BHF-UCL.
 GO:0003684; F:damaged DNA binding; IDA:UniProtKB.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IDA:BHF-UCL.
 GO:0000014; F:single-stranded DNA specific endodeoxyribonuclease activity; IMP:UniProtKB.
 GO:0001835; P:blastocyst hatching; IEA:Compara.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0010792; P:DNA double-strand break processing involved in repair via single-strand annealing; IMP:UniProtKB.
 GO:0000724; P:double-strand break repair via homologous recombination; IDA:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0031572; P:G2 DNA damage checkpoint; IDA:UniProtKB.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW. 
Interpro
 IPR019518; CtIP_N.
 IPR013882; DNA-repair_Sae2/CtIP. 
Pfam
 PF10482; CtIP_N
 PF08573; SAE2 
SMART
  
PROSITE
  
PRINTS