CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011667
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myotonin-protein kinase 
Protein Synonyms/Alias
 MT-PK; DM-kinase; DMK; DM1 protein kinase; DMPK; Myotonic dystrophy protein kinase 
Gene Name
 DMPK 
Gene Synonyms/Alias
 DM1PK; MDPK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
75RDDFEILKVIGRGAFubiquitination[1]
197GYVHRDIKPDNILLDubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity. 
Sequence Annotation
 DOMAIN 71 339 Protein kinase.
 DOMAIN 340 415 AGC-kinase C-terminal.
 NP_BIND 77 85 ATP (By similarity).
 ACT_SITE 195 195 Proton acceptor (By similarity).
 BINDING 100 100 ATP.
 MOD_RES 216 216 Phosphoserine; by autocatalysis (By
 MOD_RES 228 228 Phosphoserine; by autocatalysis (By
 MOD_RES 234 234 Phosphothreonine; by autocatalysis (By  
Keyword
 3D-structure; Alternative splicing; ATP-binding; Cataract; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Endoplasmic reticulum; Kinase; Magnesium; Membrane; Metal-binding; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Sarcoplasmic reticulum; Serine/threonine-protein kinase; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 629 AA 
Protein Sequence
MSAEVRLRRL QQLVLDPGFL GLEPLLDLLL GVHQELGASE LAQDKYVADF LQWAEPIVVR 60
LKEVRLQRDD FEILKVIGRG AFSEVAVVKM KQTGQVYAMK IMNKWDMLKR GEVSCFREER 120
DVLVNGDRRW ITQLHFAFQD ENYLYLVMEY YVGGDLLTLL SKFGERIPAE MARFYLAEIV 180
MAIDSVHRLG YVHRDIKPDN ILLDRCGHIR LADFGSCLKL RADGTVRSLV AVGTPDYLSP 240
EILQAVGGGP GTGSYGPECD WWALGVFAYE MFYGQTPFYA DSTAETYGKI VHYKEHLSLP 300
LVDEGVPEEA RDFIQRLLCP PETRLGRGGA GDFRTHPFFF GLDWDGLRDS VPPFTPDFEG 360
ATDTCNFDLV EDGLTAMVSG GGETLSDIRE GAPLGVHLPF VGYSYSCMAL RDSEVPGPTP 420
MELEAEQLLE PHVQAPSLEP SVSPQDETAE VAVPAAVPAA EAEAEVTLRE LQEALEEEVL 480
TRQSLSREME AIRTDNQNFA SQLREAEARN RDLEAHVRQL QERMELLQAE GATAVTGVPS 540
PRATDPPSHL DGPPAVAVGQ CPLVGPGPMH RRHLLLPARV PRPGLSEALS LLLFAVVLSR 600
AAALGCIGLV AHAGQLTAVW RRPGAARAP 629 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0031307; C:integral to mitochondrial outer membrane; IDA:UniProtKB.
 GO:0031965; C:nuclear membrane; ISS:UniProtKB.
 GO:0005640; C:nuclear outer membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0033017; C:sarcoplasmic reticulum membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0017020; F:myosin phosphatase regulator activity; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006874; P:cellular calcium ion homeostasis; ISS:UniProtKB.
 GO:0010657; P:muscle cell apoptotic process; IDA:UniProtKB.
 GO:0006998; P:nuclear envelope organization; IMP:UniProtKB.
 GO:0014853; P:regulation of excitatory postsynaptic membrane potential involved in skeletal muscle contraction; IEA:Compara.
 GO:0008016; P:regulation of heart contraction; IDA:UniProtKB.
 GO:0010830; P:regulation of myotube differentiation; ISS:UniProtKB.
 GO:0014722; P:regulation of skeletal muscle contraction by calcium ion signaling; IEA:Compara.
 GO:0002028; P:regulation of sodium ion transport; IEA:Compara.
 GO:0051823; P:regulation of synapse structural plasticity; IEA:Compara. 
Interpro
 IPR000961; AGC-kinase_C.
 IPR011009; Kinase-like_dom.
 IPR014930; Myotonic_dystrophy_kinase_coil.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF08826; DMPK_coil
 PF00069; Pkinase 
SMART
 SM00133; S_TK_X
 SM00220; S_TKc 
PROSITE
 PS51285; AGC_KINASE_CTER
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS