CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006632
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription factor 7 
Protein Synonyms/Alias
 TCF-7; T-cell-specific transcription factor 1; T-cell factor 1; TCF-1 
Gene Name
 TCF7 
Gene Synonyms/Alias
 TCF1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
52ERDLAELKSSLVNESubiquitination[1]
271AKKPTIKKPLNAFMLubiquitination[1]
317LSREEQAKYYELARKubiquitination[1]
371AFGTYPEKAAAPAPFubiquitination[1, 2, 3]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. 
Sequence Annotation
 DNA_BIND 269 337 HMG box.
 REGION 1 59 CTNNB1-binding.
 MOTIF 344 348 Nuclear localization signal (Potential).  
Keyword
 Activator; Alternative promoter usage; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 384 AA 
Protein Sequence
MPQLDSGGGG AGGGDDLGAP DELLAFQDEG EEQDDKSRDS AAGPERDLAE LKSSLVNESE 60
GAAGGAGIPG VPGAGAGARG EAEALGREHA AQRLFPDKLP EPLEDGLKAP ECTSGMYKET 120
VYSAFNLLMH YPPPSGAGQH PQPQPPLHKA NQPPHGVPQL SLYEHFNSPH PTPAPADISQ 180
KQVHRPLQTP DLSGFYSLTS GSMGQLPHTV SWFTHPSLML GSGVPGHPAA IPHPAIVPPS 240
GKQELQPFDR NLKTQAESKA EKEAKKPTIK KPLNAFMLYM KEMRAKVIAE CTLKESAAIN 300
QILGRRWHAL SREEQAKYYE LARKERQLHM QLYPGWSARD NYGKKKRRSR EKHQESTTGG 360
KRNAFGTYPE KAAAPAPFLP MTVL 384 
Gene Ontology
 GO:0005667; C:transcription factor complex; IBA:RefGenome.
 GO:0008013; F:beta-catenin binding; IDA:BHF-UCL.
 GO:0003682; F:chromatin binding; IBA:RefGenome.
 GO:0043565; F:sequence-specific DNA binding; IBA:RefGenome.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IBA:RefGenome.
 GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
 GO:0046632; P:alpha-beta T cell differentiation; IEA:Compara.
 GO:0007420; P:brain development; IBA:RefGenome.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IBA:RefGenome.
 GO:0071353; P:cellular response to interleukin-4; IDA:UniProtKB.
 GO:0048557; P:embryonic digestive tract morphogenesis; IEA:Compara.
 GO:0030538; P:embryonic genitalia morphogenesis; IEA:Compara.
 GO:0048619; P:embryonic hindgut morphogenesis; IEA:Compara.
 GO:0048699; P:generation of neurons; IBA:RefGenome.
 GO:0006955; P:immune response; TAS:ProtInc.
 GO:0021915; P:neural tube development; IEA:Compara.
 GO:0042981; P:regulation of apoptotic process; IEA:Compara.
 GO:0042127; P:regulation of cell proliferation; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IBA:RefGenome.
 GO:0033153; P:T cell receptor V(D)J recombination; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR027397; Catenin_binding_dom.
 IPR013558; CTNNB1-bd_N.
 IPR009071; HMG_box_dom.
 IPR024940; TCF/LEF. 
Pfam
 PF08347; CTNNB1_binding
 PF00505; HMG_box 
SMART
 SM00398; HMG 
PROSITE
 PS50118; HMG_BOX_2 
PRINTS