CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025990
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable cytosol aminopeptidase 
Protein Synonyms/Alias
 Leucine aminopeptidase; Leucyl aminopeptidase 
Gene Name
 pepA 
Gene Synonyms/Alias
 GK2947 
Created Date
 July 27, 2013 
Organism
 Geobacillus kaustophilus (strain HTA426) 
NCBI Taxa ID
 235909 
Lysine Modification
Position
Peptide
Type
References
54GRLSELQKEGDISAKacetylation[1]
Reference
 [1] Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus.
 Lee DW, Kim D, Lee YJ, Kim JA, Choi JY, Kang S, Pan JG.
 Proteomics. 2013 Aug;13(15):2278-82. [PMID: 23696451] 
Functional Description
 Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). 
Sequence Annotation
 ACT_SITE 283 283 By similarity.
 ACT_SITE 357 357 By similarity.
 METAL 271 271 Manganese 2 (By similarity).
 METAL 276 276 Manganese 1 (By similarity).
 METAL 276 276 Manganese 2 (By similarity).
 METAL 294 294 Manganese 2 (By similarity).
 METAL 353 353 Manganese 1 (By similarity).
 METAL 355 355 Manganese 1 (By similarity).
 METAL 355 355 Manganese 2 (By similarity).  
Keyword
 Aminopeptidase; Complete proteome; Cytoplasm; Hydrolase; Manganese; Metal-binding; Protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 503 AA 
Protein Sequence
MERDETMFTV KPLPSVEERD EALVVGLFEG ANSWSGLAGE YDARLGGRLS ELQKEGDISA 60
KRGRIATVHP FLPMGAKRLY FVGLGKKEEL TFERLREVFG KLFRALKQAK RTKAAVALDT 120
FTTEAVDGNE AAHALAEAYH LATYEFPGYK QKKSQPDYQL ESLIVYTAAE AAEIEASLFV 180
GSVYGKATNS ARTLVNTPGN LLTASDLADY AVKLANRYDF DYEVLEKEDM ERLGMGALLA 240
VNQGSKQPPK LIVLKYQGKD EWEDVIALVG KGVTFDTGGY CLKPRDSMVD MKTDMAGAAA 300
VLGAMEAIGE LRPEQNVLAV IPATDNMISG EAFKPDDVIT SLSGKTIEVK NTDAEGRLIL 360
ADAVTYAKQH GASYIIDVAT LTGGVIVALG TDKTGAMTNN EELFEQLLEA SMETGEFIWR 420
LPITENDRER VRSSKIADLN NSPGREGHAI MGGAFIGEFA EDTPWVHLDI AGTAATKKDG 480
DLGPAGATGV MVRTLTAFVE RFE 503 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004177; F:aminopeptidase activity; IEA:HAMAP.
 GO:0030145; F:manganese ion binding; IEA:HAMAP.
 GO:0008235; F:metalloexopeptidase activity; IEA:HAMAP.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR011356; Leucine_aapep/pepB.
 IPR000819; Peptidase_M17_C.
 IPR023042; Peptidase_M17_leu_NH2_pept.
 IPR008283; Peptidase_M17_N. 
Pfam
 PF00883; Peptidase_M17
 PF02789; Peptidase_M17_N 
SMART
  
PROSITE
 PS00631; CYTOSOL_AP 
PRINTS
 PR00481; LAMNOPPTDASE.