UniProt Accession

 HEM2_RAT; P06214 

Genbank Protein ID

 X04959; BC061806 

Genbank Nucleotide ID

 CAA28621.1; AAH61806.1 

Protein Name

 Delta-aminolevulinic acid dehydratase 

Protein Synonyms/Alias

 ALADH; Porphobilinogen synthase 

Gene Name

 Alad 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
87	GVPSRVPKDEQGSAA	acetylation	[1]
87	GVPSRVPKDEQGSAA	ubiquitination	[2]
184	AIKAALLKHGLGNRV	acetylation	[1]
252	GADILMVKPGLPYLD	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity). 

Sequence Annotation

 ACT_SITE 199 199 Schiff-base intermediate with substrate
 ACT_SITE 252 252 Schiff-base intermediate with substrate
 METAL 122 122 Zinc 1; catalytic (By similarity).
 METAL 124 124 Zinc 1; catalytic (By similarity).
 METAL 131 131 Zinc 2 (By similarity).
 METAL 132 132 Zinc 1; catalytic (By similarity).
 METAL 223 223 Zinc 2 (By similarity).
 BINDING 209 209 Substrate 1 (By similarity).
 BINDING 221 221 Substrate 1 (By similarity).
 BINDING 279 279 Substrate 2 (By similarity).
 BINDING 318 318 Substrate 2 (By similarity).  

Keyword

 Allosteric enzyme; Complete proteome; Direct protein sequencing; Heme biosynthesis; Lyase; Metal-binding; Porphyrin biosynthesis; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 330 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005615; C:extracellular space; IDA:RGD.
 GO:0032791; F:lead ion binding; ISS:UniProtKB.
 GO:0004655; F:porphobilinogen synthase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; ISS:UniProtKB.
 GO:0071284; P:cellular response to lead ion; IEP:RGD.
 GO:0006783; P:heme biosynthetic process; ISS:UniProtKB.
 GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0014823; P:response to activity; IEP:RGD.
 GO:0010044; P:response to aluminum ion; IEP:RGD.
 GO:0043200; P:response to amino acid stimulus; IEP:RGD.
 GO:0046685; P:response to arsenic-containing substance; IDA:RGD.
 GO:0046686; P:response to cadmium ion; IDA:RGD.
 GO:0032025; P:response to cobalt ion; IEP:RGD.
 GO:0042493; P:response to drug; IDA:RGD.
 GO:0045471; P:response to ethanol; IDA:RGD.
 GO:0070542; P:response to fatty acid; IEP:RGD.
 GO:0051384; P:response to glucocorticoid stimulus; IEP:RGD.
 GO:0009635; P:response to herbicide; IEP:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0010212; P:response to ionizing radiation; IEP:RGD.
 GO:0010039; P:response to iron ion; IEP:RGD.
 GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
 GO:0046689; P:response to mercury ion; IEP:RGD.
 GO:0051597; P:response to methylmercury; IEP:RGD.
 GO:0006979; P:response to oxidative stress; IEP:RGD.
 GO:0070541; P:response to platinum ion; IEP:RGD.
 GO:0010269; P:response to selenium ion; IEP:RGD.
 GO:0010266; P:response to vitamin B1; IEP:RGD.
 GO:0033197; P:response to vitamin E; IEP:RGD.
 GO:0010043; P:response to zinc ion; IDA:RGD. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR001731; Porphobilinogen_synth. 

Pfam

 PF00490; ALAD 

SMART

 SM01004; ALAD 

PROSITE

 PS00169; D_ALA_DEHYDRATASE 

PRINTS

 PR00144; DALDHYDRTASE.