CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021300
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 STE20-like serine/threonine-protein kinase 
Protein Synonyms/Alias
 STE20-like kinase; hSLK; CTCL tumor antigen se20-9; STE20-related serine/threonine-protein kinase; STE20-related kinase; Serine/threonine-protein kinase 2 
Gene Name
 SLK 
Gene Synonyms/Alias
 KIAA0204; STK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
179ADFGVSAKNTRTIQRubiquitination[1]
319TEEVEDGKEEDEEEEubiquitination[2]
337SLPIPASKRASSDLSubiquitination[2]
384NSKILNEKPTTDEPEubiquitination[2]
487MFENKLIKSEEIKDTsumoylation[3]
492LIKSEEIKDTILQTVubiquitination[2]
524DSEVGLTKEDTQEKLubiquitination[2]
663VVADTDQKALGSEVQubiquitination[2]
775SSFLSKTKDSGSISLubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Site-specific identification of SUMO-2 targets in cells reveals an inverted SUMOylation motif and a hydrophobic cluster SUMOylation motif.
 Matic I, Schimmel J, Hendriks IA, van Santen MA, van de Rijke F, van Dam H, Gnad F, Mann M, Vertegaal AC.
 Mol Cell. 2010 Aug 27;39(4):641-52. [PMID: 20797634] 
Functional Description
 Mediates apoptosis and actin stress fiber dissolution (By similarity). 
Sequence Annotation
 DOMAIN 34 292 Protein kinase.
 DOMAIN 875 910 UVR.
 NP_BIND 40 48 ATP (By similarity).
 ACT_SITE 155 155 Proton acceptor (By similarity).
 BINDING 63 63 ATP (By similarity).
 MOD_RES 14 14 Phosphoserine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 330 330 Phosphoserine.
 MOD_RES 344 344 Phosphoserine.
 MOD_RES 347 347 Phosphoserine.
 MOD_RES 348 348 Phosphoserine.
 MOD_RES 354 354 Phosphoserine (By similarity).
 MOD_RES 565 565 Phosphoserine.
 MOD_RES 571 571 Phosphoserine.
 MOD_RES 655 655 Phosphoserine.
 MOD_RES 777 777 Phosphoserine.
 MOD_RES 779 779 Phosphoserine.
 MOD_RES 818 818 Phosphoserine.
 MOD_RES 1097 1097 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; Apoptosis; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1235 AA 
Protein Sequence
MSFFNFRKIF KLGSEKKKKQ YEHVKRDLNP EDFWEIIGEL GDGAFGKVYK AQNKETSVLA 60
AAKVIDTKSE EELEDYMVEI DILASCDHPN IVKLLDAFYY ENNLWILIEF CAGGAVDAVM 120
LELERPLTES QIQVVCKQTL DALNYLHDNK IIHRDLKAGN ILFTLDGDIK LADFGVSAKN 180
TRTIQRRDSF IGTPYWMAPE VVMCETSKDR PYDYKADVWS LGITLIEMAE IEPPHHELNP 240
MRVLLKIAKS EPPTLAQPSR WSSNFKDFLK KCLEKNVDAR WTTSQLLQHP FVTVDSNKPI 300
RELIAEAKAE VTEEVEDGKE EDEEEETENS LPIPASKRAS SDLSIASSEE DKLSQNACIL 360
ESVSEKTERS NSEDKLNSKI LNEKPTTDEP EKAVEDINEH ITDAQLEAMT ELHDRTAVIK 420
ENEREKRPKL ENLPDTEDQE TVDINSVSEG KENNIMITLE TNIEHNLKSE EEKDQEKQQM 480
FENKLIKSEE IKDTILQTVD LVSQETGEKE ANIQAVDSEV GLTKEDTQEK LGEDDKTQKD 540
VISNTSDVIG TCEAADVAQK VDEDSAEDTQ SNDGKEVVEV GQKLINKPMV GPEAGGTKEV 600
PIKEIVEMNE IEEGKNKEQA INSSENIMDI NEEPGTTEGE EITESSSTEE MEVRSVVADT 660
DQKALGSEVQ DASKVTTQID KEKKEIPVSI KKEPEVTVVS QPTEPQPVLI PSININSDSG 720
ENKEEIGSLS KTETILPPES ENPKENDNDS GTGSTADTSS IDLNLSISSF LSKTKDSGSI 780
SLQETRRQKK TLKKTRKFIV DGVEVSVTTS KIVTDSDSKT EELRFLRRQE LRELRFLQKE 840
EQRAQQQLNS KLQQQREQIF RRFEQEMMSK KRQYDQEIEN LEKQQKQTIE RLEQEHTNRL 900
RDEAKRIKGE QEKELSKFQN MLKNRKKEVI NEVEKAPKEL RKELMKRRKE ELAQSQHAQE 960
QEFVQKQQQE LDGSLKKIIQ QQKAELANIE RECLNNKQQL MRAREAAIWE LEERHLQEKH 1020
QLLKQQLKDQ YFMQRHQLLK RHEKETEQMQ RYNQRLIEEL KNRQTQERAR LPKIQRSEAK 1080
TRMAMFKKSL RINSTATPDQ DRDKIKQFAA QEEKRQKNER MAQHQKHENQ MRDLQLQCEA 1140
NVRELHQLQN EKCHLLVEHE TQKLKELDEE HSQELKEWRE KLRPRKKTLE EEFARKLQEQ 1200
EVFFKMTGES ECLNPSTQSR ISKFYPIPSL HSTGS 1235 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR022165; PKK.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR001943; UVR_dom. 
Pfam
 PF00069; Pkinase
 PF12474; PKK 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50151; UVR 
PRINTS