CPLM-015357

Genbank Nucleotide ID

Protein Synonyms/Alias

Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin

Mus musculus (Mouse)

Position	Peptide	Type	References
800	EMDKLLDKISELEGN	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha- adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1. Required for hepatocyte growth factor (HGF)-induced cell migration (By similarity).

DOMAIN 496 584 PDZ.
REGION 1 154 Actin-binding (By similarity).
REGION 100 371 Interacts with D(2) dopamine receptor (By
REGION 164 282 Actin-binding (By similarity).
REGION 169 255 Interacts with ADRA2A, ADRA2B and ADRA2C
REGION 417 494 Interacts with protein phosphatase 1 (By
REGION 480 525 Interacts with RGS2 (By similarity).
REGION 595 816 Interacts with TGN38 (By similarity).
MOTIF 575 578 PP1-binding motif.
MOD_RES 15 15 Phosphoserine; by MAPK1.
MOD_RES 17 17 Phosphoserine; by CDK5.
MOD_RES 94 94 Phosphoserine; by PKA.
MOD_RES 100 100 Phosphoserine (By similarity).
MOD_RES 116 116 Phosphoserine (By similarity).
MOD_RES 192 192 Phosphoserine (By similarity).
MOD_RES 205 205 Phosphoserine; by MAPK1.

Actin-binding; Alternative splicing; Cell junction; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Membrane; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Synapse.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005912; C:adherens junction; IEA:UniProtKB-SubCell.
GO:0030864; C:cortical actin cytoskeleton; IDA:MGI.
GO:0043197; C:dendritic spine; IEA:UniProtKB-SubCell.
GO:0030175; C:filopodium; IDA:MGI.
GO:0030027; C:lamellipodium; ISS:UniProtKB.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0032587; C:ruffle membrane; ISS:UniProtKB.
GO:0045202; C:synapse; IEA:UniProtKB-SubCell.
GO:0003779; F:actin binding; TAS:MGI.
GO:0007015; P:actin filament organization; IPI:MGI.
GO:0019722; P:calcium-mediated signaling; IMP:MGI.
GO:0016477; P:cell migration; ISS:UniProtKB.
GO:0071315; P:cellular response to morphine; IMP:UniProtKB.
GO:0016358; P:dendrite development; IMP:MGI.
GO:0046847; P:filopodium assembly; ISS:UniProtKB.
GO:2000474; P:regulation of opioid receptor signaling pathway; IMP:UniProtKB.
GO:0001932; P:regulation of protein phosphorylation; IMP:MGI.