CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018601
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Myosin-7 
Protein Synonyms/Alias
 Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta 
Gene Name
 Myh7 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
67TAETENGKTVTVKEDubiquitination[1]
206AAIGDRSKKDQTPGKubiquitination[2]
207AIGDRSKKDQTPGKGubiquitination[2]
213KKDQTPGKGTLEDQIubiquitination[2]
549KATDMTFKAKLYDNHacetylation[3]
633GADAPADKGKGKAKKacetylation[3]
1106ALGSQLQKKLKELQAubiquitination[1]
1107LGSQLQKKLKELQARubiquitination[1]
1109SQLQKKLKELQARIEubiquitination[1]
1279DLTSQRAKLQTENGEubiquitination[1]
1305ISQLTRGKLTYTQQLubiquitination[1]
1503ETFKRENKNLQEEISubiquitination[1]
1531HELEKIRKQLEAEKLubiquitination[2]
1537RKQLEAEKLELQSALubiquitination[2]
1644AEAQKQVKSLQSLLKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] A proteomics approach to identify the ubiquitinated proteins in mouse heart.
 Jeon HB, Choi ES, Yoon JH, Hwang JH, Chang JW, Lee EK, Choi HW, Park ZY, Yoo YJ.
 Biochem Biophys Res Commun. 2007 Jun 8;357(3):731-6. [PMID: 17451654]
 [3] HDAC3-dependent reversible lysine acetylation of cardiac myosin heavy chain isoforms modulates their enzymatic and motor activity.
 Samant SA, Courson DS, Sundaresan NR, Pillai VB, Tan M, Zhao Y, Shroff SG, Rock RS, Gupta MP.
 J Biol Chem. 2011 Feb 18;286(7):5567-77. [PMID: 21177250] 
Functional Description
 Muscle contraction (By similarity). 
Sequence Annotation
 DOMAIN 1 780 Myosin head-like.
 DOMAIN 781 810 IQ.
 NP_BIND 178 185 ATP.
 REGION 655 677 Actin-binding.
 REGION 757 771 Actin-binding.
 MOD_RES 129 129 N6,N6,N6-trimethyllysine (Potential).
 CROSSLNK 207 207 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 213 213 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1531 1531 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1537 1537 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Isopeptide bond; Methylation; Motor protein; Muscle protein; Myosin; Nucleotide-binding; Reference proteome; Thick filament; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1935 AA 
Protein Sequence
MADAEMAAFG AAAPFLRKSE KERLEAQTRP FDLKKDVFVP DDKEEFVKAK IVSREGGKVT 60
AETENGKTVT VKEDQVMQQN PPKFDKIEDM AMLTFLHEPA VLYNLKERYA SWMIYTYSGL 120
FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES 180
GAGKTVNTKR VIQYFAVIAA IGDRSKKDQT PGKGTLEDQI IQANPALEAF GNAKTVRNDN 240
SSRFGKFIRI HFGATGKLAS ADIETYLLEK SRVIFQLKAE RDYHIFYQIL SNKKPELLDM 300
LLITNNPYDY AFISQGETTV ASIDDSEELM ATDSAFDVLG FTPEEKNSIY KLTGAIMHFG 360
NMKFKQKQRE EQAEPDGTEE ADKSAYLMGL NSADLLKGLC HPRVKVGNEY VTKGQNVQQV 420
SYAIGALAKS VYEKMFNWMV TRINATLETK QPRQYFIGVL DIAGFEIFDF NSFEQLCINF 480
TNEKLQQFFN HHMFVLEQEE YKKEGIEWTF IDFGMDLQAC IDLIEKPMGI MSILEEECMF 540
PKATDMTFKA KLYDNHLGKS NNFQKPRNVK GKQEAHFSLV HYAGTVDYNI LGWLQKNKDP 600
LNETVVGLYQ KSSLKLLSNL FANYAGADAP ADKGKGKAKK GSSFQTVSAL HRENLNKLMT 660
NLRSTHPHFV RCIIPNETKS PGVMDNPLVM HQLRCNGVLE GIRICRKGFP NRILYGDFRQ 720
RYRILNPAAI PEGQFIDSRK GAEKLLGSLD IDHNQYKFGH TKVFFKAGLL GLLEEMRDER 780
LSRIITRIQA QSRGVLSRME FKKLLERRDS LLIIQWNIRA FMGVKNWPWM KLYFKIKPLL 840
KSAETEKEMA TMKEEFGRVK DALEKSEARR KELEEKMVSL LQEKNDLQLQ VQAEQDNLAD 900
AEERCDQLIK NKIQLEAKVK EMTERLEDEE EMNAELTAKK RKLEDECSEL KRDIDDLELT 960
LAKVEKEKHA TENKVKNLTE EMAGLDEIIV KLTKEKKALQ EAHQQALDDL QAEEDKVNTL 1020
TKAKVKLEQQ VDDLEGSLEQ EKKVRMDLER AKRKLEGDLK LTQESIMDLE NDKQQLDERL 1080
KKKDFELNAL NARIEDEQAL GSQLQKKLKE LQARIEELEE ELEAERTARA KVEKLRSDLS 1140
RELEEISERL EEAGGATSVQ IEMNKKREAE FQKMRRDLEE ATLQHEATAA ALRKKHADSV 1200
AELGEQIDNL QRVKQKLEKE KSEFKLELDD VTSNMEQIIK AKANLEKMCR TLEDQMNEHR 1260
SKAEETQRSV NDLTSQRAKL QTENGELSRQ LDEKEALISQ LTRGKLTYTQ QLEDLKRQLE 1320
EEVKAKNALA HALQSARHDC DLLREQYEEE TEAKAELQRV LSKANSEVAQ WRTKYETDAI 1380
QRTEELEEAK KKLAQRLQDA EEAVEAVNAK CSSLEKTKHR LQNEIEDLMV DVERSNAAAA 1440
ALDKKQRNFD KILAEWKQKY EESQSELESS QKEARSLSTE LFKLKNAYEE SLEHLETFKR 1500
ENKNLQEEIS DLTEQLGSTG KSIHELEKIR KQLEAEKLEL QSALEEAEAS LEHEEGKILR 1560
AQLEFNQIKA EIERKLAEKD EEMEQAKRNH LRMVDSLQTS LDAETRSRNE ALRVKKKMEG 1620
DLNEMEIQLS HANRMAAEAQ KQVKSLQSLL KDTQIQLDDA VRANDDLKEN IAIVERRNNL 1680
LQAELEELRA VVEQTERSRK LAEQELIETS ERVQLLHSQN TSLINQKKKM DADLSQLQTE 1740
VEEAVQECRN AEEKAKKAIT DAAMMAEELK KEQDTSAHLE RMKKNMEQTI KDLQHRLDEA 1800
EQIALKGGKK QLQKLEARVR ELENELEAEQ KRNAESVKGM RKSERRIKEL TYQTEEDRKN 1860
LLRLQDLVDK LQLKVKAYKR QAEEAEEQAN TNLSKFRKVQ HELDEAEERA DIAESQVNKL 1920
RAKSRDIGAK GLNEE 1935 
Gene Ontology
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0016459; C:myosin complex; IDA:MGI.
 GO:0032982; C:myosin filament; IEA:UniProtKB-KW.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0001725; C:stress fiber; IDA:MGI.
 GO:0030018; C:Z disc; IDA:MGI.
 GO:0030898; F:actin-dependent ATPase activity; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003774; F:motor activity; IEA:InterPro.
 GO:0008307; F:structural constituent of muscle; IEA:Compara.
 GO:0007512; P:adult heart development; IEA:Compara.
 GO:0030049; P:muscle filament sliding; IEA:Compara.
 GO:0002027; P:regulation of heart rate; IEA:Compara.
 GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; IEA:Compara. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027401; Myosin-like_IQ_dom.
 IPR001609; Myosin_head_motor_dom.
 IPR004009; Myosin_N.
 IPR002928; Myosin_tail.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF02736; Myosin_N
 PF01576; Myosin_tail_1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.