CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011406
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polyamine transporter 3 
Protein Synonyms/Alias
  
Gene Name
 TPO3 
Gene Synonyms/Alias
 YPR156C; P9584.7 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
46NMAPNQLKLTRTETVubiquitination[1]
54LTRTETVKSLQDMGVubiquitination[1]
64QDMGVSSKAPVPDVNubiquitination[1]
137LESDNNEKGKEGDSSubiquitination[1, 2]
379RKETGNPKIMTEQEAubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermine, but not spermidine. 
Sequence Annotation
 MOD_RES 55 55 Phosphoserine.
 MOD_RES 98 98 Phosphothreonine.
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 132 132 Phosphoserine.  
Keyword
 Antiport; Cell membrane; Complete proteome; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 622 AA 
Protein Sequence
MNRQESINSF NSDETSSLSD VESQQPQQYI PSESGSKSNM APNQLKLTRT ETVKSLQDMG 60
VSSKAPVPDV NAPQSSKNKI FPEEYTLETP TGLVPVATLH SIGRTSTAIS RTRTRQIDGA 120
SSPSSNEDAL ESDNNEKGKE GDSSGANDEA PDLDPEIEFV TFVTGDPENP HNWPAWIRWS 180
YTVLLSILVI CVAYGSACIS GGLGTVEKKY HVGMEAAILS VSLMVIGFSL GPLIWSPVSD 240
LYGRRVAYFV SMGLYVIFNI PCALAPNLGS LLACRFLCGV WSSSGLCLVG GSIADMFPSE 300
TRGKAIAFFA FAPYVGPVVG PLVNGFISVS TGRMDLIFWV NMAFAGVMWI ISSAIPETYA 360
PVILKRKAAR LRKETGNPKI MTEQEAQGVS MGEMMRACLL RPLYFSVTEP VLVATCFYVC 420
LIYSLLYAFF FAFPVIFGEL YGYKDNLVGL MFIPIVIGAL WALATTFYCE NKYLQIVKQR 480
KPTPEDRLLG AKIGAPFAAI ALWILGATAY KHIIWVGPAS AGLAFGFGMV LIYYSLNNYI 540
IDCYVQYASS ALATKVFLRS AGGAAFPLFT IQMYHKLNLH WGSWLLAFIS TAMIALPFAF 600
SYWGKGLRHK LSKKDYSIDS IE 622 
Gene Ontology
 GO:0000329; C:fungal-type vacuole membrane; IMP:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
 GO:0000297; F:spermine transmembrane transporter activity; IMP:SGD. 
Interpro
 IPR011701; MFS.
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt. 
Pfam
 PF07690; MFS_1 
SMART
  
PROSITE
 PS50850; MFS 
PRINTS